An analysis of an axonal gradient of phosphorylated MAP 1B in cultured rat sensory neurons

TY - JOUR

T1 - An analysis of an axonal gradient of phosphorylated MAP 1B in cultured rat sensory neurons

AU - Bush, M S

AU - Goold, R G

AU - Moya, F

AU - Gordon-Weeks, P R

PY - 1996/2

Y1 - 1996/2

N2 - The present study investigated the cellular distribution of a developmentally regulated phosphorylated form of MAP 1B recognized by monoclonal antibody (mAb) 150 in cultures of dorsal root ganglia. The cell soma and the whole axon, when it first appears, are labelled, but longer axons label with a proximodistal gradient, such that the cell soma and proximal axon become unlabelled, whilst the distal axon and growth cone label strongly. Double-labelling experiments with mAb 150 and a polyclonal antibody (N1-15) that recognizes all forms of MAP 1B demonstrated that MAP 1B is distributed along the entire length of axons with gradients, so the gradient of phosphorylated MAP 1B is not due to a loss or absence of MAP 1B from the proximal axon. The proportion of axons from 20 h cultures that were labelled with a mAb 150 gradient was at least 80% and this proportion was independent of the nerve growth factor concentration of the culture medium. Analysis of axons ranging in length from 100 to 700 microm and labelled with a gradient showed that the unlabelled proximal portions of axons increased in length more slowly than the labelled distal axon. Axons labelled along their entire length accounted for no more than 19% of th axonal population and analysis of these showed them to be frequently

AB - The present study investigated the cellular distribution of a developmentally regulated phosphorylated form of MAP 1B recognized by monoclonal antibody (mAb) 150 in cultures of dorsal root ganglia. The cell soma and the whole axon, when it first appears, are labelled, but longer axons label with a proximodistal gradient, such that the cell soma and proximal axon become unlabelled, whilst the distal axon and growth cone label strongly. Double-labelling experiments with mAb 150 and a polyclonal antibody (N1-15) that recognizes all forms of MAP 1B demonstrated that MAP 1B is distributed along the entire length of axons with gradients, so the gradient of phosphorylated MAP 1B is not due to a loss or absence of MAP 1B from the proximal axon. The proportion of axons from 20 h cultures that were labelled with a mAb 150 gradient was at least 80% and this proportion was independent of the nerve growth factor concentration of the culture medium. Analysis of axons ranging in length from 100 to 700 microm and labelled with a gradient showed that the unlabelled proximal portions of axons increased in length more slowly than the labelled distal axon. Axons labelled along their entire length accounted for no more than 19% of th axonal population and analysis of these showed them to be frequently

KW - Animals

KW - Axons

KW - Cells, Cultured

KW - Detergents

KW - Ganglia, Spinal

KW - Microscopy, Fluorescence

KW - Microscopy, Immunoelectron

KW - Microtubule-Associated Proteins

KW - Nerve Growth Factors

KW - Nerve Tissue Proteins

KW - Neurons, Afferent

KW - Phosphorylation

KW - Protein Processing, Post-Translational

KW - Rats

KW - Rats, Wistar

M3 - Article

C2 - 8714695

SN - 0953-816X

VL - 8

SP - 235

EP - 248

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

IS - 2

ER -