An Application of Molecular Genotyping in Mice

TY - JOUR

T1 - An Application of Molecular Genotyping in Mice

AU - Underkoffler, L A

AU - Collins, J N

AU - Choi, J D

AU - Oakey, R J

PY - 2003

Y1 - 2003

N2 - Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different strains of mice have variable CA repeat lengths and PCR based methods can be used to identify them, thus allowing for specific genotypes to be assigned. Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied. We studied chromosomal segregation in relation to nondisjunction in early-gestation mouse embryos using molecular genotyping. Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

AB - Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different strains of mice have variable CA repeat lengths and PCR based methods can be used to identify them, thus allowing for specific genotypes to be assigned. Molecular genotyping offers such identification at any developmental stage, which allows for a broad range of anomalies to be studied. We studied chromosomal segregation in relation to nondisjunction in early-gestation mouse embryos using molecular genotyping. Information on the parental origin as well as the number of chromosomes a given progeny carried was obtained in our analysis.

U2 - 10.1251/bpo53

DO - 10.1251/bpo53

M3 - Article

VL - 5

SP - 116

EP - 122

JO - BIOLOGICAL PROCEDURES ONLINE

JF - BIOLOGICAL PROCEDURES ONLINE

IS - 1

ER -