An artificial di-iron oxo-protein with phenol oxidase activity

TY - JOUR

T1 - An artificial di-iron oxo-protein with phenol oxidase activity

AU - Faiella, Marina

AU - Andreozzi, Concetta

AU - Torres Martin De Rosales, Rafael

AU - Pavone, Vincenzo

AU - Maglio, Ornella

AU - Nastri, Flavia

AU - DeGrado, William F.

AU - Lombardi, Angela

PY - 2009/12

Y1 - 2009/12

N2 - Here we report the de novo design and NMR structure of a four-helical bundle di-iron protein with phenol oxidase activity. The introduction of the cofactor-binding and phenol-binding sites required the incorporation of residues that were detrimental to the free energy of folding of the protein. Sufficient stability was, however, obtained by optimizing the sequence of a loop distant from the active site.

AB - Here we report the de novo design and NMR structure of a four-helical bundle di-iron protein with phenol oxidase activity. The introduction of the cofactor-binding and phenol-binding sites required the incorporation of residues that were detrimental to the free energy of folding of the protein. Sufficient stability was, however, obtained by optimizing the sequence of a loop distant from the active site.

U2 - 10.1038/nchembio.257

DO - 10.1038/nchembio.257

M3 - Article

SN - 1552-4450

VL - 5

SP - 882

EP - 884

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 12

ER -