An automated, high-throughput method for targeted quantification of intact insulin and its therapeutic analogs in human serum or plasma coupling mass spectrometric immunoassay with high resolution and accurate mass detection (MSIA-HR/AM)

Scott Peterman*, Eric E. Niederkofler, David A. Phillips, Bryan Krastins, Urban A. Kiernan, Kemmons A. Tubbs, Dobrin Nedelkov, Amol Prakash, Maryann S. Vogelsang, Tara Schoeder, Lewis Couchman, David R Taylor, Caje Moniz, Gouri Vadali, Gregory Byram, Mary F. Lopez

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    The detection and quantification of insulin and its therapeutic analogs is important for medical, sports doping, and forensic applications. Synthetic variants contain slight sequence variations to affect bioavailability. To reduce sample handling bias, a universal extraction method is required for simultaneous extraction of endogenous and variant insulins with subsequent targeted quantification by LC-MS. A mass spectrometric immunoassay (MSIA), a multiplexed assay for intact insulin and its analogues that couples immunoenrichment with high resolution and accurate mass (HR/AM) spectrometric detection across the clinical range is presented in this report. The assay is sensitive, selective, semi-automated and can potentially be applied to detect new insulin isoforms allowing their further incorporation into second or third generation assays.

    Original languageEnglish
    Pages (from-to)1445-1456
    Number of pages12
    JournalProteomics
    Volume14
    Issue number12
    DOIs
    Publication statusPublished - Jun 2014

    Keywords

    • Diabetes
    • High resolution accurate mass
    • Insulin
    • Mass spectrometry
    • Targeted assay
    • Technology
    • CROSS-REACTIVITY
    • ASSAYS
    • STANDARDIZATION
    • VARIANTS

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