An in situ study investigating dentine tubule occlusion of dentifrices following acid challenge

Ryan Olley, Peter Pilecki, Nathan Hughes, Peter Jeffery, Rupert S. Austin, Rebecca Moazzez, David Bartlett

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


Objectives: To investigate the dentine occlusion and acid resistance of dentifrices developed to treat dentine hypersensitivity.

Methods: This was a single centre, single blind, randomised, split mouth, four treatments, two period crossover, in situ study in healthy subjects. Subjects wore buccal intra-oral appliances each fitted with four dentine samples over four consecutive days with one study product applied per appliance; 8% strontium acetate in silica base, 1040 ppm sodium fluoride (Sensodyne (R) Rapid Relief), 8% arginine, calcium carbonate, 1450 ppm sodium monofluorophosphate (Colgate Sensitive Pro-Relief (R)), 1450 ppm sodium fluoride (control paste) and water. On days 3 and 4, two agitated grapefruit juice challenges (ex vivo) occurred for 1 min. At the end of each treatment day 1 dentine sample was removed from each appliance for scanning electron microscopy (SEM). The extent of tubule occlusion was measured using an examiner-based visual scoring index (three trained examiners).

Results: In total, 28 subjects ((12 males and 16 females with a mean age of 34.7 years (SD 8.41 years)) completed the study. On day 2, both test dentifrices demonstrated significantly better dentine tubule occlusion than water (p < 0.0001) and control paste (8% strontium p = 0.0003 and 8% arginine p = 0.0019). After 3 and 4 days of twice daily brushing with acid challenges on days 3 and 4 the strontium-based dentifrice demonstrated significantly better dentine occlusion than all other treatments (p < 0.0001).

Conclusions: Strontium acetate and arginine-based dentifrice result in statistically significant dentine tubular occlusion compared to controls, but the arginine-based dentifrice is more susceptible to acid challenge.

Clinical significance: Erosive beverages are an important aetiology in DH by exposing dentine tubules. Their consumption has increased significantly over the past decade in the UK. This 4-day in situ study investigated the properties of commercially available dentifrices designed to occlude dentine tubules and their resistance to an agitated acid challenge. (C) 2012 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)585-593
Number of pages9
JournalJournal of Dentistry
Issue number7
Publication statusPublished - Jul 2012


  • Acetates
  • Adult
  • Arginine
  • Beverages
  • Calcium Carbonate
  • Carbonated Beverages
  • Carbonic Acid
  • Citric Acid
  • Citrus paradisi
  • Citrus sinensis
  • Cross-Over Studies
  • Dentifrices
  • Dentin
  • Dentin Desensitizing Agents
  • Female
  • Fluorides
  • Humans
  • Male
  • Microscopy, Electron, Scanning
  • Phosphates
  • Single-Blind Method
  • Sodium Fluoride
  • Strontium


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