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An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process

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An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. / Mesnage, Robin; Agapito-Tenfen, Sarah Z.; Vilperte, Vinicius et al.

In: Scientific Reports, Vol. 6, 37855, 19.12.2016.

Research output: Contribution to journalArticlepeer-review

Harvard

Mesnage, R, Agapito-Tenfen, SZ, Vilperte, V, Renney, G, Ward, M, Séralini, GE, Nodari, RO & Antoniou, MN 2016, 'An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process', Scientific Reports, vol. 6, 37855. https://doi.org/10.1038/srep37855

APA

Mesnage, R., Agapito-Tenfen, S. Z., Vilperte, V., Renney, G., Ward, M., Séralini, G. E., Nodari, R. O., & Antoniou, M. N. (2016). An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. Scientific Reports, 6, [37855]. https://doi.org/10.1038/srep37855

Vancouver

Mesnage R, Agapito-Tenfen SZ, Vilperte V, Renney G, Ward M, Séralini GE et al. An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. Scientific Reports. 2016 Dec 19;6. 37855. https://doi.org/10.1038/srep37855

Author

Mesnage, Robin ; Agapito-Tenfen, Sarah Z. ; Vilperte, Vinicius et al. / An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process. In: Scientific Reports. 2016 ; Vol. 6.

Bibtex Download

@article{dc5738b103574a54b42b25b3a6d85f14,
title = "An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process",
abstract = "Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as 'substantially equivalent' to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.",
author = "Robin Mesnage and Agapito-Tenfen, {Sarah Z.} and Vinicius Vilperte and George Renney and Malcolm Ward and S{\'e}ralini, {Gilles Eric} and Nodari, {Rubens O.} and Antoniou, {Michael N.}",
year = "2016",
month = dec,
day = "19",
doi = "10.1038/srep37855",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process

AU - Mesnage, Robin

AU - Agapito-Tenfen, Sarah Z.

AU - Vilperte, Vinicius

AU - Renney, George

AU - Ward, Malcolm

AU - Séralini, Gilles Eric

AU - Nodari, Rubens O.

AU - Antoniou, Michael N.

PY - 2016/12/19

Y1 - 2016/12/19

N2 - Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as 'substantially equivalent' to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.

AB - Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as 'substantially equivalent' to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.

UR - http://www.scopus.com/inward/record.url?scp=85006482097&partnerID=8YFLogxK

U2 - 10.1038/srep37855

DO - 10.1038/srep37855

M3 - Article

C2 - 27991589

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 37855

ER -

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