Abstract
The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, we describe using the Dunn chemotaxis chamber to study the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement for a specific protein in cell migration and chemotaxis.
Original language | English |
---|---|
Article number | N/A |
Pages (from-to) | 31-41 |
Number of pages | 11 |
Journal | Methods in Molecular Biology |
Volume | 294 |
Issue number | N/A |
DOIs | |
Publication status | Published - 2005 |