Analysis of cell migration using the Dunn chemotaxis chamber and time-lapse microscopy

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30 Citations (Scopus)

Abstract

The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, we describe using the Dunn chemotaxis chamber to study the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement for a specific protein in cell migration and chemotaxis.
Original languageEnglish
Article numberN/A
Pages (from-to)31-41
Number of pages11
JournalMethods in Molecular Biology
Volume294
Issue numberN/A
DOIs
Publication statusPublished - 2005

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