Analysis of microscopic parameters of single-particle trajectories in neurons

TY - JOUR

T1 - Analysis of microscopic parameters of single-particle trajectories in neurons

AU - Burlakov, V M

AU - Taylor, R

AU - Koerner, J

AU - Emptage, N

N1 - Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

PY - 2010/9/8

Y1 - 2010/9/8

N2 - We performed a comparative study of the statistical uncertainties that arise when calculating the velocity and diffusion coefficients from single-particle trajectories. We show that a method where particle mean displacement is used to calculate velocity and mean square fluctuation is used to calculate diffusion coefficient offers greater accuracy than analysis of time-dependent mean square displacement. Our assessment of the performance of the two analysis strategies is conducted in two ways. First, we apply each of the methods to simulated trajectories where each parameter term is known. Second, we analyze the motion of previously uncharacterized EphB2 receptors in the membrane of hippocampal neurons. We find that EphB2 receptors display different types of motion mode and transition between these modes. We present our data as a distribution of microscopic diffusion coefficients for each particle trajectory, which we refer to as partial distributions. Partial distributions are summed to form a cumulative distribution of diffusion coefficients for EphB2 receptors in hippocampal neurons. The structure and interpretation of the EphB2 cumulative distribution are discussed.

AB - We performed a comparative study of the statistical uncertainties that arise when calculating the velocity and diffusion coefficients from single-particle trajectories. We show that a method where particle mean displacement is used to calculate velocity and mean square fluctuation is used to calculate diffusion coefficient offers greater accuracy than analysis of time-dependent mean square displacement. Our assessment of the performance of the two analysis strategies is conducted in two ways. First, we apply each of the methods to simulated trajectories where each parameter term is known. Second, we analyze the motion of previously uncharacterized EphB2 receptors in the membrane of hippocampal neurons. We find that EphB2 receptors display different types of motion mode and transition between these modes. We present our data as a distribution of microscopic diffusion coefficients for each particle trajectory, which we refer to as partial distributions. Partial distributions are summed to form a cumulative distribution of diffusion coefficients for EphB2 receptors in hippocampal neurons. The structure and interpretation of the EphB2 cumulative distribution are discussed.

KW - Algorithms

KW - Diffusion

KW - Hippocampus/cytology

KW - Models, Biological

KW - Movement

KW - Neurons/metabolism

KW - Quantum Dots

KW - Receptor, EphB2/metabolism

KW - Time Factors

U2 - 10.1016/j.bpj.2010.06.021

DO - 10.1016/j.bpj.2010.06.021

M3 - Article

C2 - 20816048

SN - 0006-3495

VL - 99

SP - 1368

EP - 1376

JO - Biophysical Journal

JF - Biophysical Journal

IS - 5

ER -