Analysis of the in vivo assembly pathway of eukaryotic 40S ribosomal proteins

Sébastien Ferreira-Cerca, Gisela Pöll, Holger Kühn, Andreas Neueder, Steffen Jakob, Herbert Tschochner, Philipp Milkereit

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)

Abstract

In eukaryotes, in vivo formation of the two ribosomal subunits from four ribosomal RNAs (rRNAs) and approximately 80 ribosomal proteins (r-proteins) involves more than 150 nonribosomal proteins and around 100 small noncoding RNAs. It is temporally and spatially organized within different cellular compartments: the nucleolus, the nucleoplasm, and the cytoplasm. Here, we present a way to analyze how eukaryotic r-proteins of the small ribosomal subunit (SSU) assemble in vivo with rRNA. Our results show that key aspects of the assembly of eukaryotic r-proteins into distinct structural parts of the SSU are similar to the in vitro assembly pathway of their prokaryotic counterparts. We observe that the establishment of a stable assembly intermediate of the eukaryotic SSU body, but not of the SSU head, is closely linked to early rRNA processing events. The formation of assembly intermediates of the head controls efficient nuclear export of the SSU and cytoplasmic pre-rRNA maturation steps.
Original languageEnglish
Pages (from-to)446-57
Number of pages12
JournalMOLECULAR CELL
Volume28
Issue number3
DOIs
Publication statusPublished - 9 Nov 2007

Keywords

  • Models, Biological
  • RNA Precursors
  • Ribosomal Proteins
  • Ribosome Subunits, Small, Eukaryotic
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins

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