Angiogenesis selectively requires the p110 alpha isoform of PI3K to control endothelial cell migration

TY - JOUR

T1 - Angiogenesis selectively requires the p110 alpha isoform of PI3K to control endothelial cell migration

AU - Graupera, M

AU - Guillermet-Guibert, J

AU - Foukas, L C

AU - Phng, L K

AU - Cain, R J

AU - Salpekar, A

AU - Pearce, W

AU - Meek, S

AU - Millan, J

AU - Cutillas, P R

AU - Smith, A J H

AU - Ridley, A J

AU - Ruhrberg, C

AU - Gerhardt, H

AU - Vanhaesebroeck, B

PY - 2008/5/29

Y1 - 2008/5/29

N2 - Phosphoinositide 3- kinases ( PI3Ks) signal downstream of multiple cell- surface receptor types. Class IA PI3K isoforms(1) couple to tyrosine kinases and consist of a p110 catalytic subunit ( p110 alpha, p110 beta or p110 delta), constitutively bound to one of five distinct p85 regulatory subunits. PI3Ks have been implicated in angiogenesis(2-5), but little is known about potential selectivity among the PI3K isoforms and their mechanism of action in endothelial cells during angiogenesis in vivo. Here we show that only p110 alpha activity is essential for vascular development. Ubiquitous or endothelial cell- specific inactivation of p110 alpha led to embryonic lethality at mid- gestation because of severe defects in angiogenic sprouting and vascular remodelling. p110 alpha exerts this critical endothelial cell- autonomous function by regulating endothelial cell migration through the small GTPase RhoA. p110 alpha activity is particularly high in endothelial cells and preferentially induced by tyrosine kinase ligands ( such as vascular endothelial growth factor ( VEGF)- A). In contrast, p110 delta in endothelial cells signals downstream of G- protein- coupled receptor ( GPCR) ligands such as SDF-1 alpha, whereas p110 delta is expressed at low level and contributes only minimally to PI3K activity in endothelial cells. These results provide the first in vivo evidence for p110- isoform

AB - Phosphoinositide 3- kinases ( PI3Ks) signal downstream of multiple cell- surface receptor types. Class IA PI3K isoforms(1) couple to tyrosine kinases and consist of a p110 catalytic subunit ( p110 alpha, p110 beta or p110 delta), constitutively bound to one of five distinct p85 regulatory subunits. PI3Ks have been implicated in angiogenesis(2-5), but little is known about potential selectivity among the PI3K isoforms and their mechanism of action in endothelial cells during angiogenesis in vivo. Here we show that only p110 alpha activity is essential for vascular development. Ubiquitous or endothelial cell- specific inactivation of p110 alpha led to embryonic lethality at mid- gestation because of severe defects in angiogenic sprouting and vascular remodelling. p110 alpha exerts this critical endothelial cell- autonomous function by regulating endothelial cell migration through the small GTPase RhoA. p110 alpha activity is particularly high in endothelial cells and preferentially induced by tyrosine kinase ligands ( such as vascular endothelial growth factor ( VEGF)- A). In contrast, p110 delta in endothelial cells signals downstream of G- protein- coupled receptor ( GPCR) ligands such as SDF-1 alpha, whereas p110 delta is expressed at low level and contributes only minimally to PI3K activity in endothelial cells. These results provide the first in vivo evidence for p110- isoform

U2 - 10.1038/nature06892

DO - 10.1038/nature06892

M3 - Article

SN - 1476-4687

VL - 453

SP - 662 - 6U9

JO - NATURE

JF - NATURE

IS - 7195

ER -