Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae)

TY - JOUR

T1 - Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae)

AU - Moody, J O

AU - Robert, V A

AU - Connolly, J D

AU - Houghton, P J

PY - 2006/3/8

Y1 - 2006/3/8

N2 - Sphenocentrum jollyanum crude extracts and an isolated constituent were evaluated for anti-inflammatory activity using the carrageenan-induced hind paw oedema of healthy adult albino rats and utilizing the oral route of administration. The fruit methanol extract (79.58% inhibition at 200 mg kg(-1)) gave a higher anti-inflammatory activity than the root extract (53.75% inhibition at 200 mg ml(-1)). Further purification of the most active fruit methanol extract (MFE) led to the isolation of three furanoditerpenes identified as columbin, isocolumbin, fibleucin (uv, ir, nmr and ms) as well as a flavonoid-rich fraction (FDE). Both columbin (67.08% inhibition at 20 mg kg(-1), p <0.05) and FDE (76.25% inhibition at 200 mg kg(-1); p <0.05) gave significant anti-inflammatory activities in comparable range with reference acetylsalicylic acid (72.50% inhibition at 100 mg kg(-1)). The results provide some justification for the folkloric uses of Sphenocentrum jollyanum in the treatment of inflammatory-based diseases across the West African sub-region. (c) 2005 Elsevier Ireland Ltd. All rights reserved

AB - Sphenocentrum jollyanum crude extracts and an isolated constituent were evaluated for anti-inflammatory activity using the carrageenan-induced hind paw oedema of healthy adult albino rats and utilizing the oral route of administration. The fruit methanol extract (79.58% inhibition at 200 mg kg(-1)) gave a higher anti-inflammatory activity than the root extract (53.75% inhibition at 200 mg ml(-1)). Further purification of the most active fruit methanol extract (MFE) led to the isolation of three furanoditerpenes identified as columbin, isocolumbin, fibleucin (uv, ir, nmr and ms) as well as a flavonoid-rich fraction (FDE). Both columbin (67.08% inhibition at 20 mg kg(-1), p <0.05) and FDE (76.25% inhibition at 200 mg kg(-1); p <0.05) gave significant anti-inflammatory activities in comparable range with reference acetylsalicylic acid (72.50% inhibition at 100 mg kg(-1)). The results provide some justification for the folkloric uses of Sphenocentrum jollyanum in the treatment of inflammatory-based diseases across the West African sub-region. (c) 2005 Elsevier Ireland Ltd. All rights reserved

U2 - 10.1016/j.jep.2005.08.051

DO - 10.1016/j.jep.2005.08.051

M3 - Article

VL - 104

SP - 87

EP - 91

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

IS - 1-2

ER -