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Antibiotic-in-cyclodextrin-in-liposomes: formulation development and interactions with model bacterial membranes

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Kalliopi-Kelli A. Vandera, Pietro Picconi, Margarita Valero, Gustavo González-Gaitano, Arcadia Woods, Nur Masirah Zain, Kenneth Bruce, Luke A. Clifton, Maximilian W.A. Skoda, Khondaker Miraz Rahman, Richard D. Harvey, Cecile Dreiss

Original languageEnglish
Pages (from-to)2354-2369
Number of pages16
JournalMolecular Pharmaceutics
Volume17
Issue number7
Early online date30 Apr 2020
DOIs
Publication statusPublished - 6 Jul 2020

King's Authors

Abstract

Gram-negative bacteria possess numerous defenses against antibiotics, due to the intrinsic permeability barrier of their outer membrane (OM), explaining the recalcitrance of some common and life-threatening infections. We report the formulation of a new drug, PPA148, which shows promising activity against all Gram-negative bacteria included in the ESKAPEE pathogens. PPA148 was solubilized by inclusion complexation with cyclodextrin followed by encapsulation in liposomes. The complex and liposomal formulation presented increased activity against E. coli compared to the pure drug when assessed with the Kirby Bauer assay. The novel formulation containing 1 μg PPA148 reached similar efficacy levels equivalent to those of 30 μg of pure rifampicin. A range of biophysical techniques was used to explore the mechanism of drug uptake. Langmuir trough (LT) and neutron reflectivity (NR) techniques were employed to monitor the interactions between the drug and the formulation with model membranes. We found evidence for liposome fusion with the model Gram-negative outer membrane and for cyclodextrins acting as inner membrane (IM) permeation enhancers without presenting intrinsic antimicrobial activity. An antibiotic-in-cyclodextrin-in-liposomes (ACL) formulation was developed, which targets both the bacterial OM and IM, and offers promise as a means to breach the Gram-negative cell envelope.

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