Applications of ATR-FTIR spectroscopic imaging to biomedical samples

S. G. Kazarian*, K. L. A. Chan

*Corresponding author for this work

Research output: Contribution to journalLiterature reviewpeer-review

309 Citations (Scopus)

Abstract

FTIR spectroscopic imaging in ATR (Attenuated Total Reflection) mode is a powerful tool for studying biomedical samples. This paper summarises recent advances in the applications of ATR-FTIR imaging to dissolution of pharmaceutical formulations and drug release. The use of two different ATR accessories to obtain chemical images of formulations in contact with water as a function of time is demonstrated. The innovative use of the diamond ATR accessory allowed in situ imaging of tablet compaction and dissolution. ATR-FTIR imaging was also applied to obtain images of the surface of skin and the spatial distribution of protein and lipid rich domains was obtained. Chemical images of cross-section of rabbit aorta were obtained using a diamond ATR accessory and the possibility of in situ imaging of arterial samples in contact with aqueous solution was demonstrated for the first time. This experiment opens an opportunity to image arterial samples in contact with solutions containing drug molecules. This approach may help in understanding the mechanisms of treatment of atherosclerosis.

Original languageEnglish
Article numberN/A
Pages (from-to)858-867
Number of pages10
JournalBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume1758
Issue number7
DOIs
Publication statusPublished - Jul 2006

Keywords

  • FT-IR spectroscopic imaging
  • infrared spectroscopy
  • drug release
  • dissolution
  • skin
  • aorta
  • SOLVENT-INDUCED CRYSTALLIZATION
  • POLYMER DISSOLUTION
  • HIGH-THROUGHPUT
  • DRUG-RELEASE
  • PHARMACEUTICAL FORMULATIONS
  • INFRARED-SPECTROSCOPY
  • SPATIAL-RESOLUTION
  • STRATUM-CORNEUM
  • IR SPECTROSCOPY
  • LIQUID-CRYSTALS

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