Arachidonic acid-dependent gene regulation during preadipocyte differentiation controls adipocyte potential

Evanthia Nikolopoulou, Georgia Papacleovoulou, Frederic Jean-Alphonse, Giulia Grimaldi, Malcolm G. Parker, Aylin C. Hanyaloglu, Mark Christian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Arachidonic acid (AA) is a major PUFA that has been implicated in the regulation of adipogenesis. We examined the effect of a short exposure to AA at different stages of 3T3-L1 adipocyte differentiation. AA caused the upregulation of fatty acid binding protein 4 (FABP4/aP2) following 24 h of differentiation. This was mediated by the prostaglandin F (PGF ), as inhibition of cyclooxygenases or PGF receptor signaling counteracted the AA-mediated aP2 induction. In addition, calcium, protein kinase C, and ERK are all key elements of the pathway through which AA induces the expression of aP2. We also show that treatment with AA during the fi rst 24 h of differentiation upregulates the expression of the transcription factor Fos-related antigen 1 (Fra-1) via the same pathway. Finally, treatment with AA for 24 h at the beginning of the adipocyte differentiation is suffi cient to inhibit the late stages of adipogenesis through a Fra-1-dependent pathway, as Fra-1 knockdown rescued adipogenesis. Our data show that AA is able to program the differentiation potential of preadipocytes by regulating gene expression at the early stages of adipogenesis.

Original languageEnglish
Pages (from-to)2479-2490
Number of pages12
JournalJournal of Lipid Research
Issue number12
Publication statusPublished - Dec 2014


  • Adipogenesis
  • Cell signaling
  • Metabolism
  • Prostaglandins


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