Artifact-free high-density localization microscopy analysis

Richard J. Marsh; Karin Pfisterer; Pauline Bennett; Liisa M Hirvonen; Mathias Gautel; Gareth E. Jones; Susan Cox

doi:10.1038/s41592-018-0072-5

Artifact-free high-density localization microscopy analysis

Richard J. Marsh, Karin Pfisterer, Pauline Bennett, Liisa M Hirvonen, Mathias Gautel, Gareth E. Jones, Susan Cox

King's College London

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

637 Downloads (Pure)

Abstract

High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

Original language	English
Pages (from-to)	689-692
Journal	NATURE METHODS
Volume	15
Early online date	30 Jul 2018
DOIs	https://doi.org/10.1038/s41592-018-0072-5
Publication status	Published - Sept 2018

Access to Document

10.1038/s41592-018-0072-5

Artefact-free high density_MARSH_Accepted8June2018_GREEN AAMAccepted author manuscript, 637 KB
Artefact-free high density SUPP INFO_MARSH_Accepted8June2018_GREEN AAMAccepted author manuscript, 3.07 MB

Raw data for HAWK paper
Marsh, R., Pfisterer, K., Bennett, P., Hirvonen, L., Gautel, M., Jones, G. E. & Cox, S., King's College London, 5 Jun 2018
DOI: 10.18742/RDM01-318, https://kcl.figshare.com/articles/dataset/Raw_data_for_HAWK_paper/16473801
Dataset

Cite this

@article{a30467a9551e479f85fbfe0ee672a3a4,

title = "Artifact-free high-density localization microscopy analysis",

abstract = "High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.",

author = "Marsh, {Richard J.} and Karin Pfisterer and Pauline Bennett and Hirvonen, {Liisa M} and Mathias Gautel and Jones, {Gareth E.} and Susan Cox",

year = "2018",

month = sep,

doi = "10.1038/s41592-018-0072-5",

language = "English",

volume = "15",

pages = "689--692",

journal = "NATURE METHODS",

issn = "1548-7091",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Artifact-free high-density localization microscopy analysis

AU - Marsh, Richard J.

AU - Pfisterer, Karin

AU - Bennett, Pauline

AU - Hirvonen, Liisa M

AU - Gautel, Mathias

AU - Jones, Gareth E.

AU - Cox, Susan

PY - 2018/9

Y1 - 2018/9

N2 - High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

AB - High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

U2 - 10.1038/s41592-018-0072-5

DO - 10.1038/s41592-018-0072-5

M3 - Article

C2 - 30061677

SN - 1548-7091

VL - 15

SP - 689

EP - 692

JO - NATURE METHODS

JF - NATURE METHODS

ER -

Artifact-free high-density localization microscopy analysis

Abstract

Access to Document

Fingerprint

Datasets

Raw data for HAWK paper

Cite this