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Artifact-free high-density localization microscopy analysis

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)689-692
JournalNATURE METHODS
Volume15
Early online date30 Jul 2018
DOIs
Publication statusPublished - Sep 2018

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King's Authors

Abstract

High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

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