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Artifact-free high-density localization microscopy analysis

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)689-692
Early online date30 Jul 2018
Accepted/In press8 Jun 2018
E-pub ahead of print30 Jul 2018
PublishedSep 2018


King's Authors


High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

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