King's College London

Research portal

Artificial oral fluid characterisation: Potential for use as a reference matrix in drug testing

Research output: Contribution to journalArticle

Ivana Gavrilović, Alessandro Musenga, David Cowan, Alison Woffendin, Andrew Smart, Fan Gong, Duncan Harding, Kim Wolff

Original languageEnglish
JournalDrug Testing And Analysis
DOIs
Accepted/In press2020

King's Authors

Abstract

Quality assurance schemes for drug-screening programmes require access to large quantities of biological matrices for reference or control samples. This presents problems when the availability of a matrix, such as oral fluid (OF) for screening or for confirmatory purposes, limits the collection of large volumes. In such cases, synthetic alternatives of OF may provide a solution. The preparation of an artificial (synthetic) oral fluid (AOF) was conducted by dissolving its components (salts, surfactant, antimicrobial agent and mucin) in water. We characterised the physical properties of AOF to determine its suitability as a matrix for quality assurance purposes. The evaluation of pH, specific gravity (SG), conductivity (mS cm−1), freezing point depression (°C), light-scattering and kinematic viscosity (mm2 s−1) showed AOF to be a stable, reliable matrix. Synthetic OF was prepared using components (mucin, surfactants and so on) obtained from different suppliers and a comparison was performed. Our results suggest that AOF is a feasible matrix for the preparation of quality assurance samples for confirmatory or drug screening programmes.

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454