Artificial oral fluid characterisation: Potential for use as a reference matrix in drug testing

Ivana Gavrilović; Alessandro Musenga; David Cowan; Alison Woffendin; Andrew Smart; Fan Gong; Duncan Harding; Kim Wolff

doi:10.1002/dta.2938

Artificial oral fluid characterisation: Potential for use as a reference matrix in drug testing

Ivana Gavrilović^*, Alessandro Musenga, David Cowan, Alison Woffendin, Andrew Smart, Fan Gong, Duncan Harding, Kim Wolff

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Quality assurance schemes for drug-screening programmes require access to large quantities of biological matrices for reference or control samples. This presents problems when the availability of a matrix, such as oral fluid (OF) for screening or for confirmatory purposes, limits the collection of large volumes. In such cases, synthetic alternatives of OF may provide a solution. The preparation of an artificial (synthetic) oral fluid (AOF) was conducted by dissolving its components (salts, surfactant, antimicrobial agent and mucin) in water. We characterised the physical properties of AOF to determine its suitability as a matrix for quality assurance purposes. The evaluation of pH, specific gravity (SG), conductivity (mS cm⁻¹), freezing point depression (°C), light-scattering and kinematic viscosity (mm² s⁻¹) showed AOF to be a stable, reliable matrix. Synthetic OF was prepared using components (mucin, surfactants and so on) obtained from different suppliers and a comparison was performed. Our results suggest that AOF is a feasible matrix for the preparation of quality assurance samples for confirmatory or drug screening programmes.

Original language	English
Number of pages	11
Journal	Drug Testing And Analysis
Volume	13
Issue number	3
Early online date	18 Oct 2020
DOIs	https://doi.org/10.1002/dta.2938
Publication status	E-pub ahead of print - 18 Oct 2020

Keywords

AOF
drug screening
quality assurance samples
reference matrix

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10.1002/dta.2938

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abstract = "Quality assurance schemes for drug-screening programmes require access to large quantities of biological matrices for reference or control samples. This presents problems when the availability of a matrix, such as oral fluid (OF) for screening or for confirmatory purposes, limits the collection of large volumes. In such cases, synthetic alternatives of OF may provide a solution. The preparation of an artificial (synthetic) oral fluid (AOF) was conducted by dissolving its components (salts, surfactant, antimicrobial agent and mucin) in water. We characterised the physical properties of AOF to determine its suitability as a matrix for quality assurance purposes. The evaluation of pH, specific gravity (SG), conductivity (mS cm−1), freezing point depression (°C), light-scattering and kinematic viscosity (mm2 s−1) showed AOF to be a stable, reliable matrix. Synthetic OF was prepared using components (mucin, surfactants and so on) obtained from different suppliers and a comparison was performed. Our results suggest that AOF is a feasible matrix for the preparation of quality assurance samples for confirmatory or drug screening programmes.",

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AU - Gavrilović, Ivana

AU - Musenga, Alessandro

AU - Cowan, David

AU - Woffendin, Alison

AU - Smart, Andrew

AU - Gong, Fan

AU - Harding, Duncan

AU - Wolff, Kim

PY - 2020/10/18

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N2 - Quality assurance schemes for drug-screening programmes require access to large quantities of biological matrices for reference or control samples. This presents problems when the availability of a matrix, such as oral fluid (OF) for screening or for confirmatory purposes, limits the collection of large volumes. In such cases, synthetic alternatives of OF may provide a solution. The preparation of an artificial (synthetic) oral fluid (AOF) was conducted by dissolving its components (salts, surfactant, antimicrobial agent and mucin) in water. We characterised the physical properties of AOF to determine its suitability as a matrix for quality assurance purposes. The evaluation of pH, specific gravity (SG), conductivity (mS cm−1), freezing point depression (°C), light-scattering and kinematic viscosity (mm2 s−1) showed AOF to be a stable, reliable matrix. Synthetic OF was prepared using components (mucin, surfactants and so on) obtained from different suppliers and a comparison was performed. Our results suggest that AOF is a feasible matrix for the preparation of quality assurance samples for confirmatory or drug screening programmes.

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Artificial oral fluid characterisation: Potential for use as a reference matrix in drug testing

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Keywords

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