Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex

TY - JOUR

T1 - Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex

AU - Thumser, Alfred E.

AU - Rashed, Aswir Abd

AU - Sharp, Paul A.

AU - Lodge, John K.

PY - 2010/11/15

Y1 - 2010/11/15

N2 - It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration-dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+-ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+-ascorbate complex. (C) 2010 Elsevier Ltd. All rights reserved.

AB - It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration-dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+-ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+-ascorbate complex. (C) 2010 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.foodchem.2010.04.031

DO - 10.1016/j.foodchem.2010.04.031

M3 - Article

VL - 123

SP - 281

EP - 285

JO - FOOD CHEMISTRY

JF - FOOD CHEMISTRY

IS - 2

ER -