ATR-FTIR spectroscopic imaging: recent advances and applications to biological systems

Sergei G. Kazarian*, K. L. Andrew Chan

*Corresponding author for this work

Research output: Contribution to journalLiterature reviewpeer-review

306 Citations (Scopus)

Abstract

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic imaging is a highly versatile, label free and non-destructive chemical imaging method which can be applied to study a wide range of samples and systems. This review summarises some of the recent advances and applications of this imaging method in the area of biomedical studies, including examples of section of aorta, skin tissue and live cells. Two of the major advantages of measuring in ATR mode are the opportunity to measure samples that absorb strongly in the IR spectrum, such as aqueous systems, without significant sample preparation and the ability to increase the spatial resolution of the measured image. The implications of these advantages as well as some limitations of this imaging approach are discussed and a brief outlook at some of the possible future developments in this area is provided.

Original languageEnglish
Article numberN/A
Pages (from-to)1940-1951
Number of pages12
JournalAnalyst
Volume138
Issue number7
DOIs
Publication statusPublished - 7 Apr 2013

Keywords

  • EMBRYONIC STEM-CELLS
  • INFRARED-SPECTROSCOPY
  • REFLECTION SPECTROSCOPY
  • PROTEIN CRYSTALLIZATION
  • SPATIAL-RESOLUTION
  • IR SPECTROSCOPY
  • VARIABLE ANGLES
  • DRUG-RELEASE
  • MICRO
  • TISSUE

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