Attachment of stem cells to scaffold particles for intra-cerebral transplantation

Ellen Bible; David Y. S. Chau; Morgan R. Alexander; Jack Price; Kevin M. Shakesheff; Michel Modo

doi:10.1038/nprot.2009.156

Attachment of stem cells to scaffold particles for intra-cerebral transplantation

Ellen Bible, David Y. S. Chau, Morgan R. Alexander, Jack Price, Kevin M. Shakesheff, Michel Modo

University of Nottingham

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.

Original language	English
Pages (from-to)	1440 - 1453
Number of pages	14
Journal	Nature Protocols
Volume	4
Issue number	10
DOIs	https://doi.org/10.1038/nprot.2009.156
Publication status	Published - 2009

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abstract = "Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.",

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Attachment of stem cells to scaffold particles for intra-cerebral transplantation

Abstract

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