B cell antigen extraction is regulated by physical properties of antigen-presenting cells

Katelyn M. Spillane, Pavel Tolar

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)
169 Downloads (Pure)

Abstract

Antibody production and affinity maturation are driven by B cell extraction and internalization of antigen from immune synapses. However, the extraction mechanism remains poorly understood. Here we develop DNA-based nanosensors to interrogate two previously proposed mechanisms, enzymatic liberation and mechanical force. Using antigens presented by either artificial substrates or live cells, we show that B cells primarily use force-dependent extraction and resort to enzymatic liberation only if mechanical forces fail to retrieve antigen. The use of mechanical forces renders antigen extraction sensitive to the physical properties of the presenting cells. We show that follicular dendritic cells are stiff cells that promote strong B cell pulling forces and stringent affinity discrimination. In contrast, dendritic cells are soft and promote acquisition of low-affinity antigens through low forces. Thus, the mechanical properties of B cell synapses regulate antigen extraction, suggesting that distinct properties of presenting cells support different stages of B cell responses.
Original languageEnglish
Pages (from-to)217-230
JournalJournal of Cell Biology
Volume216
Issue number1
Early online date6 Dec 2016
DOIs
Publication statusPublished - 6 Dec 2016

Fingerprint

Dive into the research topics of 'B cell antigen extraction is regulated by physical properties of antigen-presenting cells'. Together they form a unique fingerprint.

Cite this