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Banff 2019 Meeting Report: Molecular diagnostics in solid organ transplantation-Consensus for the Banff Human Organ Transplant (B-HOT) gene panel and open source multicenter validation

Research output: Contribution to journalArticle

Michael Mengel, Alexandre Loupy, Mark Haas, Candice Roufosse, Maarten Naesens, Enver Akalin, Marian C. Clahsen-van Groningen, Jessy Dagobert, Anthony J. Demetris, Jean Paul Duong van Huyen, Juliette Gueguen, Fadi Issa, Blaise Robin, Ivy Rosales, Jan H. Von der Thüsen, Alberto Sanchez-Fueyo, Rex N. Smith, Kathryn Wood, Benjamin Adam, Robert B. Colvin

Original languageEnglish
Pages (from-to)2305-2317
Number of pages13
JournalAmerican Journal of Transplantation
Volume20
Issue number9
DOIs
Publication statusPublished - 1 Sep 2020

King's Authors

Abstract

This meeting report from the XV Banff conference describes the creation of a multiorgan transplant gene panel by the Banff Molecular Diagnostics Working Group (MDWG). This Banff Human Organ Transplant (B-HOT) panel is the culmination of previous work by the MDWG to identify a broadly useful gene panel based on whole transcriptome technology. A data-driven process distilled a gene list from peer-reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, and lung transplant biopsies. These were supplemented by genes that define relevant cellular pathways and cell types plus 12 reference genes used for normalization. The 770 gene B-HOT panel includes the most pertinent genes related to rejection, tolerance, viral infections, and innate and adaptive immune responses. This commercially available panel uses the NanoString platform, which can quantitate transcripts from formalin-fixed paraffin-embedded samples. The B-HOT panel will facilitate multicenter collaborative clinical research using archival samples and permit the development of an open source large database of standardized analyses, thereby expediting clinical validation studies. The MDWG believes that a pathogenesis and pathway based molecular approach will be valuable for investigators and promote therapeutic decision-making and clinical trials.

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