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BDNF and its pro-peptide are stored in presynaptic large dense core vesicles in brain neurons.

Research output: Contribution to journalArticle

Sandra Dieni, Tomoya Matsumoto, Martijn Dekkers, Stefanie Rauskolb, Mihai S. Ionescu, Ruben Deogracias Pastor, Eckart D Gundelfinger, Masami Kojima, Sigrun Nestel, Michael Frotscher, Yves-Alain Barde

Original languageEnglish
Pages (from-to)775-788
JournalJ Cell Biol
Volume196
Issue number6
DOIs
Publication statusPublished - 19 Mar 2012

King's Authors

Abstract

Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ∼10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.

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