Abstract
The availability of cryopreserved hepatocytes is required for a more widespread use of hepatocyte transplantation, but human hepatocytes are easily damaged during freezing-thawing. Here, preincubation with unconjugated bilirubin, a physiological antioxidant, resulted in increased viability and function of hepatocytes (as determined by trypan blue exclusion, mitochondrial succinate dehydrogenases activity, urea synthesis, and cytochrome P450 1A/2) compared with cells incubated without the pigment. These findings suggest that unconjugated bilirubin may be used as cryoprotectant in clinical hepatocyte transplantation.
Original language | English |
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Pages (from-to) | 691 - 693 |
Number of pages | 3 |
Journal | Journal of Pediatric Gastroenterology and Nutrition |
Volume | 50 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2010 |