Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window

Struan Bourke; Federico Dona; Yurema Teijeiro Gonzalez; Basma Qazi Chaudhry; Maryna Panamarova; Eirinn Mackay; Peter S. Zammit; Lea Ann Dailey; Ulrike S. Eggert; Klaus Suhling; Mark A. Green

doi:10.1021/acsapm.2c01153

Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window

Struan Bourke, Federico Dona, Yurema Teijeiro Gonzalez, Basma Qazi Chaudhry, Maryna Panamarova, Eirinn Mackay, Peter S. Zammit, Lea Ann Dailey, Ulrike S. Eggert, Klaus Suhling, Mark A. Green

UCL University College London

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Abstract

Conjugated polymers are organic semiconductors that can be used for fluorescence microscopy of living specimens. Here, we report the encapsulation of the bright-red-emitting conjugated polymer, poly[{9,9-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene}-alt-co-{2,5-bis(N,N′-diphenylamino)-1,4-phenylene}] (CN-FO-DPD), and superparamagnetic iron oxide nanoparticles (SPIONs) within poly(styrene-co-maleic anhydride) (PSMA) micelles. The resulting particles exhibited an emission peak at 657 nm, a fluorescence quantum yield of 21%, an average diameter of 65 nm, and a ζ potential of -30 mV. They are taken up by cells, and we describe their use in fluorescence microscopy of living Hela cells and zebrafish embryos and their associated cytotoxicity in HEK, HeLa, and HCE cells.

Original language	English
Pages (from-to)	8193–8202
Number of pages	10
Journal	ACS Applied Polymer Materials
Volume	4
Issue number	11
Early online date	12 Oct 2022
DOIs	https://doi.org/10.1021/acsapm.2c01153
Publication status	Published - 11 Nov 2022

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Biocompatible Magnetic Conjugated Polymer_BOURKE_Publishedonline12October2022_GOLD VoR (CC BY)Final published version, 9.61 MBLicence: CC BY

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Bourke, S., Dona, F., Teijeiro Gonzalez, Y., Qazi Chaudhry, B., Panamarova, M., Mackay, E., Zammit, P. S., Dailey, L. A., Eggert, U. S., Suhling, K., & Green, M. A. (2022). Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window. ACS Applied Polymer Materials, 4(11), 8193–8202. https://doi.org/10.1021/acsapm.2c01153

@article{90f6937dc31e472fab24fd658dda40cf,

title = "Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window",

abstract = "Conjugated polymers are organic semiconductors that can be used for fluorescence microscopy of living specimens. Here, we report the encapsulation of the bright-red-emitting conjugated polymer, poly[{9,9-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene}-alt-co-{2,5-bis(N,N′-diphenylamino)-1,4-phenylene}] (CN-FO-DPD), and superparamagnetic iron oxide nanoparticles (SPIONs) within poly(styrene-co-maleic anhydride) (PSMA) micelles. The resulting particles exhibited an emission peak at 657 nm, a fluorescence quantum yield of 21%, an average diameter of 65 nm, and a ζ potential of -30 mV. They are taken up by cells, and we describe their use in fluorescence microscopy of living Hela cells and zebrafish embryos and their associated cytotoxicity in HEK, HeLa, and HCE cells.",

author = "Struan Bourke and Federico Dona and {Teijeiro Gonzalez}, Yurema and {Qazi Chaudhry}, Basma and Maryna Panamarova and Eirinn Mackay and Zammit, {Peter S.} and Dailey, {Lea Ann} and Eggert, {Ulrike S.} and Klaus Suhling and Green, {Mark A.}",

note = "Funding Information: The authors thank the Nikon Imaging Centre at King{\textquoteright}s College London light microscopy for both live and fixed samples. They also thank Dr. Richard Thorogate at the London Centre of Nanotechnology for his assistance in analyzing the magnetic behavior of the CPNs containing SPIONs. They acknowledge ERC Starting (Consolidator) Grant 306659 (U.E., F.D.) and Wellcome Investigator Award 110060/Z/15/Z (U.E.) Publisher Copyright: {\textcopyright} 2022 ACS Applied Polymer Materials. All right reserved.",

year = "2022",

month = nov,

day = "11",

doi = "10.1021/acsapm.2c01153",

language = "English",

volume = "4",

pages = "8193–8202",

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Bourke, S , Dona, F , Teijeiro Gonzalez, Y , Qazi Chaudhry, B , Panamarova, M, Mackay, E, Zammit, PS , Dailey, LA , Eggert, US , Suhling, K & Green, MA 2022, 'Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window', ACS Applied Polymer Materials, vol. 4, no. 11, pp. 8193–8202. https://doi.org/10.1021/acsapm.2c01153

TY - JOUR

T1 - Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window

AU - Bourke, Struan

AU - Dona, Federico

AU - Teijeiro Gonzalez, Yurema

AU - Qazi Chaudhry, Basma

AU - Panamarova, Maryna

AU - Mackay, Eirinn

AU - Zammit, Peter S.

AU - Dailey, Lea Ann

AU - Eggert, Ulrike S.

AU - Suhling, Klaus

AU - Green, Mark A.

N1 - Funding Information: The authors thank the Nikon Imaging Centre at King’s College London light microscopy for both live and fixed samples. They also thank Dr. Richard Thorogate at the London Centre of Nanotechnology for his assistance in analyzing the magnetic behavior of the CPNs containing SPIONs. They acknowledge ERC Starting (Consolidator) Grant 306659 (U.E., F.D.) and Wellcome Investigator Award 110060/Z/15/Z (U.E.) Publisher Copyright: © 2022 ACS Applied Polymer Materials. All right reserved.

PY - 2022/11/11

Y1 - 2022/11/11

N2 - Conjugated polymers are organic semiconductors that can be used for fluorescence microscopy of living specimens. Here, we report the encapsulation of the bright-red-emitting conjugated polymer, poly[{9,9-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene}-alt-co-{2,5-bis(N,N′-diphenylamino)-1,4-phenylene}] (CN-FO-DPD), and superparamagnetic iron oxide nanoparticles (SPIONs) within poly(styrene-co-maleic anhydride) (PSMA) micelles. The resulting particles exhibited an emission peak at 657 nm, a fluorescence quantum yield of 21%, an average diameter of 65 nm, and a ζ potential of -30 mV. They are taken up by cells, and we describe their use in fluorescence microscopy of living Hela cells and zebrafish embryos and their associated cytotoxicity in HEK, HeLa, and HCE cells.

AB - Conjugated polymers are organic semiconductors that can be used for fluorescence microscopy of living specimens. Here, we report the encapsulation of the bright-red-emitting conjugated polymer, poly[{9,9-dihexyl-2,7-bis(1-cyanovinylene)fluorenylene}-alt-co-{2,5-bis(N,N′-diphenylamino)-1,4-phenylene}] (CN-FO-DPD), and superparamagnetic iron oxide nanoparticles (SPIONs) within poly(styrene-co-maleic anhydride) (PSMA) micelles. The resulting particles exhibited an emission peak at 657 nm, a fluorescence quantum yield of 21%, an average diameter of 65 nm, and a ζ potential of -30 mV. They are taken up by cells, and we describe their use in fluorescence microscopy of living Hela cells and zebrafish embryos and their associated cytotoxicity in HEK, HeLa, and HCE cells.

UR - https://pubs.acs.org/doi/full/10.1021/acsapm.2c01153

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U2 - 10.1021/acsapm.2c01153

DO - 10.1021/acsapm.2c01153

M3 - Article

SN - 2637-6105

VL - 4

SP - 8193

EP - 8202

JO - ACS Applied Polymer Materials

JF - ACS Applied Polymer Materials

IS - 11

ER -

Biocompatible magnetic conjugated polymer nanoparticles for optical and lifetime imaging applications in the first biological window

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