TY - JOUR
T1 - Bioengineering human skeletal muscle models
T2 - Recent advances, current challenges and future perspectives
AU - Jiang, Yunsong
AU - Torun, Tugce
AU - Maffioletti, Sara M.
AU - Serio, Andrea
AU - Tedesco, Francesco Saverio
N1 - Funding Information:
The authors thank Mattia Gerli for feedback on the manuscript. This work was supported by the European Research Council (759108 – HISTOID), the Francis Crick Institute , which receives its core funding from Cancer Research UK , the UK Medical Research Council , and the Welcome Trust (FC001002); Muscular Dystrophy UK (19GRO-PS48-0188; 17GRO-PS48-0093-1), the BBSRC and the NIHR (the views expressed are those of the authors and not necessarily those of the National Health Service, the NIHR, or the Department of Health ). Work on muscle tissue engineering in the Tedesco laboratory was also supported by the AFM-Telethon and Duchenne Parent Project . T.T. acknowledges support from the Ministry of National Education of the Republic of Turkey for her PhD studies. For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.
Publisher Copyright:
© 2022
PY - 2022/7/15
Y1 - 2022/7/15
N2 - Engineering models of human skeletal muscle tissue provides unique translational opportunities to investigate and develop therapeutic strategies for acute muscle injuries, and to establish personalised and precision medicine platforms for in vitro studies of severe neuromuscular and musculoskeletal disorders. Several myogenic and non-myogenic cell types can be isolated, generated, amplified and combined with scaffolds and biomaterials to achieve this aim. Novel bio-fabrication strategies, which include exogenous stimuli to enhance tissue maturation, promise to achieve an ever-increasing degree of tissue functionalisation both in vivo and in vitro. Here we review recent advances, current challenges and future perspectives to build human skeletal muscle tissue “in a dish”, focusing on the cellular constituents and on applications for in vitro disease modelling. We also briefly discuss the impact that emerging technologies such as 3D bioprinting, organ-on-chip and organoids might have to circumvent technical hurdles in future studies.
AB - Engineering models of human skeletal muscle tissue provides unique translational opportunities to investigate and develop therapeutic strategies for acute muscle injuries, and to establish personalised and precision medicine platforms for in vitro studies of severe neuromuscular and musculoskeletal disorders. Several myogenic and non-myogenic cell types can be isolated, generated, amplified and combined with scaffolds and biomaterials to achieve this aim. Novel bio-fabrication strategies, which include exogenous stimuli to enhance tissue maturation, promise to achieve an ever-increasing degree of tissue functionalisation both in vivo and in vitro. Here we review recent advances, current challenges and future perspectives to build human skeletal muscle tissue “in a dish”, focusing on the cellular constituents and on applications for in vitro disease modelling. We also briefly discuss the impact that emerging technologies such as 3D bioprinting, organ-on-chip and organoids might have to circumvent technical hurdles in future studies.
KW - 3D scaffolds
KW - Biomaterials
KW - Human skeletal muscle
KW - Myogenic cells
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85129023755&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2022.113133
DO - 10.1016/j.yexcr.2022.113133
M3 - Article
C2 - 35427601
AN - SCOPUS:85129023755
SN - 0014-4827
VL - 416
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
M1 - 113133
ER -