Blueberry intervention improves vascular reactivity and lowers blood pressure in high-fat-, high-cholesterol-fed rats

Ana Rodriguez-Mateos; Akari Ishisaka; Kazuaki Mawatari; Alberto Vidal-Diez; Jeremy P E Spencer; Junji Terao

doi:10.1017/S0007114512003911

Blueberry intervention improves vascular reactivity and lowers blood pressure in high-fat-, high-cholesterol-fed rats

Ana Rodriguez-Mateos, Akari Ishisaka, Kazuaki Mawatari, Alberto Vidal-Diez, Jeremy P E Spencer, Junji Terao

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Growing evidence suggests that intake of flavonoid-containing foods may exert cardiovascular benefits in human subjects. We have investigated the effects of a 10-week blueberry (BB) supplementation on blood pressure (BP) and vascular reactivity in rats fed a high-fat/high-cholesterol diet, known to induce endothelial dysfunction. Rats were randomly assigned to follow a control chow diet, a chow diet supplemented with 2 % (w/w) BB, a high-fat diet (10 % lard; 0·5 % cholesterol) or the high fat plus BB for 10 weeks. Rats supplemented with BB showed significant reductions in systolic BP (SBP) of 11 and 14 %, at weeks 8 and 10, respectively, relative to rats fed the control chow diet (week 8 SBP: 107·5 (SEM 4·7) v. 122·2 (SEM 2·1) mmHg, P= 0·018; week 10 SBP: 115·0 (SEM 3·1) v. 132·7 (SEM 1·5) mmHg, P< 0·0001). Furthermore, SBP was reduced by 14 % in rats fed with the high fat plus 2 % BB diet at week 10, compared to those on the high-fat diet only (SBP: 118·2 (SEM 3·6) v. 139·5 (SEM 4·5) mmHg, P< 0·0001). Aortas harvested from BB-fed animals exhibited significantly reduced contractile responses (to L-phenylephrine) compared to those fed the control chow or high-fat diets. Furthermore, in rats fed with high fat supplemented with BB, aorta relaxation was significantly greater in response to acetylcholine compared to animals fed with the fat diet. These data suggest that BB consumption can lower BP and improve endothelial dysfunction induced by a high fat, high cholesterol containing diet.

Original language	English
Pages (from-to)	1746-54
Number of pages	9
Journal	British Journal of Nutrition
Volume	109
Issue number	10
DOIs	https://doi.org/10.1017/S0007114512003911
Publication status	Published - 28 May 2013

Keywords

Acetylcholine
Animals
Aorta
Blood Pressure
Cholesterol, Dietary
Diet, High-Fat
Dietary Fats
Dietary Supplements
Endothelium, Vascular
Flavonoids
Fruit
Male
Muscle, Smooth, Vascular
Phenylephrine
Phytotherapy
Plant Preparations
Rats
Rats, Wistar
Vaccinium
Vascular Diseases
Vasoconstriction
Vasodilator Agents

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1017/S0007114512003911

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@article{d034582507764297a71e173f7c0616c6,

title = "Blueberry intervention improves vascular reactivity and lowers blood pressure in high-fat-, high-cholesterol-fed rats",

abstract = "Growing evidence suggests that intake of flavonoid-containing foods may exert cardiovascular benefits in human subjects. We have investigated the effects of a 10-week blueberry (BB) supplementation on blood pressure (BP) and vascular reactivity in rats fed a high-fat/high-cholesterol diet, known to induce endothelial dysfunction. Rats were randomly assigned to follow a control chow diet, a chow diet supplemented with 2 % (w/w) BB, a high-fat diet (10 % lard; 0·5 % cholesterol) or the high fat plus BB for 10 weeks. Rats supplemented with BB showed significant reductions in systolic BP (SBP) of 11 and 14 %, at weeks 8 and 10, respectively, relative to rats fed the control chow diet (week 8 SBP: 107·5 (SEM 4·7) v. 122·2 (SEM 2·1) mmHg, P= 0·018; week 10 SBP: 115·0 (SEM 3·1) v. 132·7 (SEM 1·5) mmHg, P< 0·0001). Furthermore, SBP was reduced by 14 % in rats fed with the high fat plus 2 % BB diet at week 10, compared to those on the high-fat diet only (SBP: 118·2 (SEM 3·6) v. 139·5 (SEM 4·5) mmHg, P< 0·0001). Aortas harvested from BB-fed animals exhibited significantly reduced contractile responses (to L-phenylephrine) compared to those fed the control chow or high-fat diets. Furthermore, in rats fed with high fat supplemented with BB, aorta relaxation was significantly greater in response to acetylcholine compared to animals fed with the fat diet. These data suggest that BB consumption can lower BP and improve endothelial dysfunction induced by a high fat, high cholesterol containing diet.",

keywords = "Acetylcholine, Animals, Aorta, Blood Pressure, Cholesterol, Dietary, Diet, High-Fat, Dietary Fats, Dietary Supplements, Endothelium, Vascular, Flavonoids, Fruit, Male, Muscle, Smooth, Vascular, Phenylephrine, Phytotherapy, Plant Preparations, Rats, Rats, Wistar, Vaccinium, Vascular Diseases, Vasoconstriction, Vasodilator Agents",

author = "Ana Rodriguez-Mateos and Akari Ishisaka and Kazuaki Mawatari and Alberto Vidal-Diez and Spencer, {Jeremy P E} and Junji Terao",

year = "2013",

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doi = "10.1017/S0007114512003911",

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journal = "British Journal of Nutrition",

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T1 - Blueberry intervention improves vascular reactivity and lowers blood pressure in high-fat-, high-cholesterol-fed rats

AU - Rodriguez-Mateos, Ana

AU - Ishisaka, Akari

AU - Mawatari, Kazuaki

AU - Vidal-Diez, Alberto

AU - Spencer, Jeremy P E

AU - Terao, Junji

PY - 2013/5/28

Y1 - 2013/5/28

N2 - Growing evidence suggests that intake of flavonoid-containing foods may exert cardiovascular benefits in human subjects. We have investigated the effects of a 10-week blueberry (BB) supplementation on blood pressure (BP) and vascular reactivity in rats fed a high-fat/high-cholesterol diet, known to induce endothelial dysfunction. Rats were randomly assigned to follow a control chow diet, a chow diet supplemented with 2 % (w/w) BB, a high-fat diet (10 % lard; 0·5 % cholesterol) or the high fat plus BB for 10 weeks. Rats supplemented with BB showed significant reductions in systolic BP (SBP) of 11 and 14 %, at weeks 8 and 10, respectively, relative to rats fed the control chow diet (week 8 SBP: 107·5 (SEM 4·7) v. 122·2 (SEM 2·1) mmHg, P= 0·018; week 10 SBP: 115·0 (SEM 3·1) v. 132·7 (SEM 1·5) mmHg, P< 0·0001). Furthermore, SBP was reduced by 14 % in rats fed with the high fat plus 2 % BB diet at week 10, compared to those on the high-fat diet only (SBP: 118·2 (SEM 3·6) v. 139·5 (SEM 4·5) mmHg, P< 0·0001). Aortas harvested from BB-fed animals exhibited significantly reduced contractile responses (to L-phenylephrine) compared to those fed the control chow or high-fat diets. Furthermore, in rats fed with high fat supplemented with BB, aorta relaxation was significantly greater in response to acetylcholine compared to animals fed with the fat diet. These data suggest that BB consumption can lower BP and improve endothelial dysfunction induced by a high fat, high cholesterol containing diet.

AB - Growing evidence suggests that intake of flavonoid-containing foods may exert cardiovascular benefits in human subjects. We have investigated the effects of a 10-week blueberry (BB) supplementation on blood pressure (BP) and vascular reactivity in rats fed a high-fat/high-cholesterol diet, known to induce endothelial dysfunction. Rats were randomly assigned to follow a control chow diet, a chow diet supplemented with 2 % (w/w) BB, a high-fat diet (10 % lard; 0·5 % cholesterol) or the high fat plus BB for 10 weeks. Rats supplemented with BB showed significant reductions in systolic BP (SBP) of 11 and 14 %, at weeks 8 and 10, respectively, relative to rats fed the control chow diet (week 8 SBP: 107·5 (SEM 4·7) v. 122·2 (SEM 2·1) mmHg, P= 0·018; week 10 SBP: 115·0 (SEM 3·1) v. 132·7 (SEM 1·5) mmHg, P< 0·0001). Furthermore, SBP was reduced by 14 % in rats fed with the high fat plus 2 % BB diet at week 10, compared to those on the high-fat diet only (SBP: 118·2 (SEM 3·6) v. 139·5 (SEM 4·5) mmHg, P< 0·0001). Aortas harvested from BB-fed animals exhibited significantly reduced contractile responses (to L-phenylephrine) compared to those fed the control chow or high-fat diets. Furthermore, in rats fed with high fat supplemented with BB, aorta relaxation was significantly greater in response to acetylcholine compared to animals fed with the fat diet. These data suggest that BB consumption can lower BP and improve endothelial dysfunction induced by a high fat, high cholesterol containing diet.

KW - Acetylcholine

KW - Animals

KW - Aorta

KW - Blood Pressure

KW - Cholesterol, Dietary

KW - Diet, High-Fat

KW - Dietary Fats

KW - Dietary Supplements

KW - Endothelium, Vascular

KW - Flavonoids

KW - Fruit

KW - Male

KW - Muscle, Smooth, Vascular

KW - Phenylephrine

KW - Phytotherapy

KW - Plant Preparations

KW - Rats

KW - Rats, Wistar

KW - Vaccinium

KW - Vascular Diseases

KW - Vasoconstriction

KW - Vasodilator Agents

U2 - 10.1017/S0007114512003911

DO - 10.1017/S0007114512003911

M3 - Article

C2 - 23046999

SN - 0007-1145

VL - 109

SP - 1746

EP - 1754

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 10

ER -

Blueberry intervention improves vascular reactivity and lowers blood pressure in high-fat-, high-cholesterol-fed rats

Abstract

Keywords

UN SDGs

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