Broadband coherent Raman imaging for multiplexed detection

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Abstract

The Raman spectrum of a fluorescent chromophore typically has many spectral features, which differ markedly between dyes even if their electronic spectra are similar. This high information content makes it possible to distinguish biomarkers based on their Raman spectra. Coherent anti-Stokes Raman scattering may therefore allow for the simultaneous measurement of more biomarkers than is possible with fluorescent imaging, while avoiding bleaching and sample autofluorescence. We have built a broadband CARS microspectrometer to demonstrate the principle of CARS multiplexing and investigate the potential to apply the system to studies of biological samples.
Original languageEnglish
Article number79032J
JournalPROCEEDINGS- SPIE THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING
Volume7903
DOIs
Publication statusPublished - 28 Feb 2011

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