Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination

TY - JOUR

T1 - Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination

AU - Kawauchi, Junya

AU - Mischo, Hannah

AU - Braglia, Priscilla

AU - Rondon, Ana

AU - Proudfoot, Nick J

PY - 2008/4/15

Y1 - 2008/4/15

N2 - Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. For two well-defined Pol II-transcribed genes, CYC1 and PMA1, we demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol II, following mRNA 3' end processing. Similarly, Pol I termination relies on prior Rnt1p cleavage at the 3' end of the pre-rRNA 35S transcript. This is followed by the combined actions of Rat1p and Sen1p to degrade the Pol I-associated nascent transcript that consequently promote termination in the downstream rDNA spacer sequence. Our data suggest that the previously defined in vitro Pol I termination mechanism involving the action of the Reb1p DNA-binding factor to "road-block" Pol I transcription close to the termination region may have overlooked more complex in vivo molecular processes.

AB - Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. For two well-defined Pol II-transcribed genes, CYC1 and PMA1, we demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol II, following mRNA 3' end processing. Similarly, Pol I termination relies on prior Rnt1p cleavage at the 3' end of the pre-rRNA 35S transcript. This is followed by the combined actions of Rat1p and Sen1p to degrade the Pol I-associated nascent transcript that consequently promote termination in the downstream rDNA spacer sequence. Our data suggest that the previously defined in vitro Pol I termination mechanism involving the action of the Reb1p DNA-binding factor to "road-block" Pol I transcription close to the termination region may have overlooked more complex in vivo molecular processes.

KW - Cytochromes c/metabolism

KW - DNA Helicases

KW - DNA, Ribosomal/metabolism

KW - Exoribonucleases/metabolism

KW - Fungal Proteins/metabolism

KW - Models, Biological

KW - Proton-Translocating ATPases/metabolism

KW - RNA Helicases

KW - RNA Polymerase I/metabolism

KW - RNA Polymerase II/metabolism

KW - Saccharomyces cerevisiae/enzymology

KW - Saccharomyces cerevisiae Proteins/metabolism

KW - Terminator Regions, Genetic/genetics

KW - Transcription, Genetic

U2 - 10.1101/gad.463408

DO - 10.1101/gad.463408

M3 - Article

C2 - 18413718

SN - 0890-9369

VL - 22

SP - 1082

EP - 1092

JO - Genes and Development

JF - Genes and Development

IS - 8

ER -