Building Proficient Enzymes with Foldamer Prostheses

Clemens Mayer; Manuel M. Mueller; Samuel H. Gellman; Donald Hilvert

doi:10.1002/anie.201400945

Building Proficient Enzymes with Foldamer Prostheses

Clemens Mayer, Manuel M. Mueller, Samuel H. Gellman, Donald Hilvert

Chemistry

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Foldamers are non‐natural oligomers that adopt stable conformations reminiscent of those found in proteins. To evaluate the potential of foldameric subunits for catalysis, semisynthetic enzymes containing foldamer fragments constructed from α‐ and β‐amino acid residues were designed and characterized. Systematic variation of the α→β substitution pattern and types of β‐residue afforded highly proficient hybrid catalysts, thus demonstrating the feasibility of expanding the enzyme‐engineering toolkit with non‐natural backbones.

Original language	English
Pages (from-to)	6978-6981
Journal	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume	53
Issue number	27
Early online date	14 May 2014
DOIs	https://doi.org/10.1002/anie.201400945
Publication status	Published - Jul 2014

Keywords

beta-amino acids
enzymes
foldamers
protein design
protein engineering

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10.1002/anie.201400945

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abstract = "Foldamers are non‐natural oligomers that adopt stable conformations reminiscent of those found in proteins. To evaluate the potential of foldameric subunits for catalysis, semisynthetic enzymes containing foldamer fragments constructed from α‐ and β‐amino acid residues were designed and characterized. Systematic variation of the α→β substitution pattern and types of β‐residue afforded highly proficient hybrid catalysts, thus demonstrating the feasibility of expanding the enzyme‐engineering toolkit with non‐natural backbones.",

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KW - beta-amino acids

KW - enzymes

KW - foldamers

KW - protein design

KW - protein engineering

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M3 - Article

SN - 1433-7851

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Building Proficient Enzymes with Foldamer Prostheses

Abstract

Keywords

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