CaMV 355 promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)

P. Arokiaraj; H. Yeet Yeang; K. Fong Cheong; S. Hamzah; H. Jones; S. Coomber; B. V. Charlwood

doi:10.1007/s002990050454

CaMV 355 promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)

P. Arokiaraj^*, H. Yeet Yeang, K. Fong Cheong, S. Hamzah, H. Jones, S. Coomber, B. V. Charlwood

^*Corresponding author for this work

King's College London

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Hevea brasiliensis anther calli were genetically transformed using Agrobacterium GV2260 (p35SGUSINT) that harboured the β-glucuronidase (gus) and neomycin phosphotransferase (nptII) genes. β-Glucuronidase protein (GUS) was expressed in the leaves of kanamycin-resistant plants that were regnerated, and the presence of the gene was confirmed by Southern analysis. GUS was also observed to be expressed in the latex and more importantly in the serum fraction. Transverse sections of the leaf petiole from a transformed plant revealed GUS expression to be especially enhanced in the phloem and laticifers. GUS expression was subsequently detected in every one of 194 plants representing three successive vegetative cycles propagated from the original transformant. Transgenic Hevea could thus facilitate the continual production of foreign proteins expressed in the latex.

Original language	English
Pages (from-to)	621-625
Number of pages	5
Journal	Plant Cell Reports
Volume	17
Issue number	8
Early online date	May 1998
DOIs	https://doi.org/10.1007/s002990050454
Publication status	E-pub ahead of print - May 1998

Keywords

β-Glucuronidase
Agrobacterium
CaMV 35S
Hevea brasiliensis
Latex

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10.1007/s002990050454

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title = "CaMV 355 promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)",

abstract = "Hevea brasiliensis anther calli were genetically transformed using Agrobacterium GV2260 (p35SGUSINT) that harboured the β-glucuronidase (gus) and neomycin phosphotransferase (nptII) genes. β-Glucuronidase protein (GUS) was expressed in the leaves of kanamycin-resistant plants that were regnerated, and the presence of the gene was confirmed by Southern analysis. GUS was also observed to be expressed in the latex and more importantly in the serum fraction. Transverse sections of the leaf petiole from a transformed plant revealed GUS expression to be especially enhanced in the phloem and laticifers. GUS expression was subsequently detected in every one of 194 plants representing three successive vegetative cycles propagated from the original transformant. Transgenic Hevea could thus facilitate the continual production of foreign proteins expressed in the latex.",

keywords = "β-Glucuronidase, Agrobacterium, CaMV 35S, Hevea brasiliensis, Latex",

author = "P. Arokiaraj and Yeang, {H. Yeet} and Cheong, {K. Fong} and S. Hamzah and H. Jones and S. Coomber and Charlwood, {B. V.}",

year = "1998",

month = may,

doi = "10.1007/s002990050454",

language = "English",

volume = "17",

pages = "621--625",

journal = "Plant Cell Reports",

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TY - JOUR

T1 - CaMV 355 promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)

AU - Arokiaraj, P.

AU - Yeang, H. Yeet

AU - Cheong, K. Fong

AU - Hamzah, S.

AU - Jones, H.

AU - Coomber, S.

AU - Charlwood, B. V.

PY - 1998/5

Y1 - 1998/5

N2 - Hevea brasiliensis anther calli were genetically transformed using Agrobacterium GV2260 (p35SGUSINT) that harboured the β-glucuronidase (gus) and neomycin phosphotransferase (nptII) genes. β-Glucuronidase protein (GUS) was expressed in the leaves of kanamycin-resistant plants that were regnerated, and the presence of the gene was confirmed by Southern analysis. GUS was also observed to be expressed in the latex and more importantly in the serum fraction. Transverse sections of the leaf petiole from a transformed plant revealed GUS expression to be especially enhanced in the phloem and laticifers. GUS expression was subsequently detected in every one of 194 plants representing three successive vegetative cycles propagated from the original transformant. Transgenic Hevea could thus facilitate the continual production of foreign proteins expressed in the latex.

AB - Hevea brasiliensis anther calli were genetically transformed using Agrobacterium GV2260 (p35SGUSINT) that harboured the β-glucuronidase (gus) and neomycin phosphotransferase (nptII) genes. β-Glucuronidase protein (GUS) was expressed in the leaves of kanamycin-resistant plants that were regnerated, and the presence of the gene was confirmed by Southern analysis. GUS was also observed to be expressed in the latex and more importantly in the serum fraction. Transverse sections of the leaf petiole from a transformed plant revealed GUS expression to be especially enhanced in the phloem and laticifers. GUS expression was subsequently detected in every one of 194 plants representing three successive vegetative cycles propagated from the original transformant. Transgenic Hevea could thus facilitate the continual production of foreign proteins expressed in the latex.

KW - β-Glucuronidase

KW - Agrobacterium

KW - CaMV 35S

KW - Hevea brasiliensis

KW - Latex

U2 - 10.1007/s002990050454

DO - 10.1007/s002990050454

M3 - Article

AN - SCOPUS:0031597138

SN - 0721-7714

VL - 17

SP - 621

EP - 625

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 8

ER -

CaMV 355 promoter directs β-glucuronidase expression in the laticiferous system of transgenic Hevea brasiliensis (rubber tree)

Abstract

Keywords

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