Candidalysin triggers epithelial cellular stresses that induce necrotic death

Mariana Blagojevic, Giorgio Camilli, Michelle Maxson, Bernhard Hube, David L. Moyes, Jonathan P. Richardson, Julian R. Naglik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
177 Downloads (Pure)


Candida albicans is a common opportunistic fungal pathogen that causes a wide range of infections from superficial mucosal to hematogenously disseminated candidiasis. The hyphal form plays an important role in the pathogenic process by invading epithelial cells and causing tissue damage. Notably, the secretion of the hyphal toxin candidalysin is essential for both epithelial cell damage and activation of mucosal immune responses. However, the mechanism of candidalysin-induced cell death remains unclear. Here, we examined the induction of cell death by candidalysin in oral epithelial cells. Fluorescent imaging using healthy/apoptotic/necrotic cell markers revealed that candidalysin causes a rapid and marked increase in the population of necrotic rather than apoptotic cells in a concentration dependent manner. Activation of a necrosis-like pathway was confirmed since C. albicans and candidalysin failed to activate caspase-8 and -3, or the cleavage of poly (ADP-ribose) polymerase. Furthermore, oral epithelial cells treated with candidalysin showed rapid production of reactive oxygen species, disruption of mitochondria activity and mitochondrial membrane potential, ATP depletion and cytochrome c release. Collectively, these data demonstrate that oral epithelial cells respond to the secreted fungal toxin candidalysin by triggering numerous cellular stress responses that induce necrotic death.

Take aways
Candidalysin secreted from Candida albicans causes epithelial cell stress.
Candidalysin induces calcium influx and oxidative stress in host cells.
Candidalysin induces mitochondrial dysfunction, ATP depletion and epithelial necrosis.
The toxicity of candidalysin is mediated from the epithelial cell surface.
Original languageEnglish
Article numbere13371
JournalCellular Microbiology
Issue number10
Early online date16 Jun 2021
Publication statusPublished - Oct 2021


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