Casein kinase I epsilon associates with and phosphorylates the tight junction protein occludin

TY - JOUR

T1 - Casein kinase I epsilon associates with and phosphorylates the tight junction protein occludin

AU - McKenzie, J A G

AU - Riento, K

AU - Ridley, A J

PY - 2006/4/17

Y1 - 2006/4/17

N2 - Occludin is an integral-membrane protein that contributes to tight junction function. We have identified casein kinase I epsilon (CKI epsilon) as a binding partner for the C-terminal cytoplasmic domain of occludin by yeast two-hybrid screening. CKI epsilon phosphorylated occludin and co-localised and co-immunoprecipitated with occludin from human endothelial cells. Amino acids 265-318 of occludin were sufficient for CKI epsilon binding and phosphorylation. Deletion of the C-terminal 48 amino acids of occludin increased CKI epsilon binding and phosphorylation, suggesting that this region inhibits CKI epsilon binding. These data identify CKI epsilon as a novel occludin kinase that may be important for the regulation of occludin. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

AB - Occludin is an integral-membrane protein that contributes to tight junction function. We have identified casein kinase I epsilon (CKI epsilon) as a binding partner for the C-terminal cytoplasmic domain of occludin by yeast two-hybrid screening. CKI epsilon phosphorylated occludin and co-localised and co-immunoprecipitated with occludin from human endothelial cells. Amino acids 265-318 of occludin were sufficient for CKI epsilon binding and phosphorylation. Deletion of the C-terminal 48 amino acids of occludin increased CKI epsilon binding and phosphorylation, suggesting that this region inhibits CKI epsilon binding. These data identify CKI epsilon as a novel occludin kinase that may be important for the regulation of occludin. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

U2 - 10.1016/j.febslet.2006.03.048

DO - 10.1016/j.febslet.2006.03.048

M3 - Article

VL - 580

SP - 2388

EP - 2394

JO - FEBS Letters

JF - FEBS Letters

IS - 9

ER -