CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope

Leandro N. Ventimiglia, Miguel Angel Cuesta-Geijo, Nicolas Martinelli, Anna Caballe, Pauline Macheboeuf, Nolwenn Miguet, Ian M. Parnham, Yolanda Olmos, Jeremy G. Carlton, Winfried Weissenhorn, Juan Martin-Serrano

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


© 2018 The Author(s) The coordinated reformation of the nuclear envelope (NE) after mitosis re-establishes the structural integrity and the functionality of the nuclear compartment. The endosomal sorting complex required for transport (ESCRT) machinery, a membrane remodeling pathway that is highly conserved in eukaryotes, has been recently involved in NE resealing by mediating the annular fusion of the nuclear membrane (NM). We show here that CC2D1B, a regulator of ESCRT polymerization, is required to re-establish the nuclear compartmentalization by coordinating endoplasmic reticulum (ER) membrane deposition around chromatin disks with ESCRT-III recruitment to the reforming NE. Accordingly, CC2D1B determines the spatiotemporal distribution of the CHMP7-ESCRT-III axis during NE reformation. Crucially, in CC2D1B-depleted cells, ESCRT activity is uncoupled from Spastin-mediated severing of spindle microtubules, resulting in persisting microtubules that compromise nuclear morphology. Therefore, we reveal CC2D1B as an essential regulatory factor that licenses the formation of ESCRT-III polymers to ensure the orderly reformation of the NE. A faithful reformation of the nuclear envelope after mitosis is critical to ensure the proper compartmentalization of the genetic material. Ventimiglia et al. identified CC2D1B as a key regulator of this process to ensure properly timed ESCRT-III machinery recruitment and licensing of ESCRT-III filaments for nuclear envelope resealing.
Original languageEnglish
Pages (from-to)547-563.e6
JournalDevelopmental Cell
Issue number5
Early online date3 Dec 2018
Publication statusE-pub ahead of print - 3 Dec 2018


  • microtubules
  • nuclear envelope
  • Spastin


Dive into the research topics of 'CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope'. Together they form a unique fingerprint.

Cite this