CD4+CD25+ T cells as immunoregulatory T cells in vitro

TY - JOUR

T1 - CD4+CD25+ T cells as immunoregulatory T cells in vitro

AU - Chai, J-G

AU - Tsang, J Y S

AU - Lechler, R

AU - Simpson, E

AU - Dyson, J

AU - Scott, D

PY - 2002/8

Y1 - 2002/8

N2 - We have further characterized the in vitro phenotype and function of anergic and suppressive CD4+25+ T cells. Following TCR ligation, DO. 11.10 CD4+25+ T cells suppress the activation of OT-1 CD8+25- T cells in an antigen nonspecific manner. Although suppression was seen when using a mixture of APC from both parental strains, it was very much more marked when using F1 APC. APC pretreated with, and then separated from CD4+25+ T cells did not have diminished T cell costimulatory function, suggesting that APC are not the direct targets of CD4+25+ T cell regulation. CTLA-4 blockade failed to abrogate suppression by CD4+25+ T cells in mixing experiments. Although CD4+25+ T cells failed to respond following cross-linking of TCR, they could be induced to proliferate following the addition of exogenous IL-2, allowing the generation of a T cell line from CD4+25+ T cells. After the first in vitro restimulation, CD4+25+ T cells were still anergic and suppressive following TCR engagement. However, after three rounds of restimulation, their anergic and suppressive status was abrogated.

AB - We have further characterized the in vitro phenotype and function of anergic and suppressive CD4+25+ T cells. Following TCR ligation, DO. 11.10 CD4+25+ T cells suppress the activation of OT-1 CD8+25- T cells in an antigen nonspecific manner. Although suppression was seen when using a mixture of APC from both parental strains, it was very much more marked when using F1 APC. APC pretreated with, and then separated from CD4+25+ T cells did not have diminished T cell costimulatory function, suggesting that APC are not the direct targets of CD4+25+ T cell regulation. CTLA-4 blockade failed to abrogate suppression by CD4+25+ T cells in mixing experiments. Although CD4+25+ T cells failed to respond following cross-linking of TCR, they could be induced to proliferate following the addition of exogenous IL-2, allowing the generation of a T cell line from CD4+25+ T cells. After the first in vitro restimulation, CD4+25+ T cells were still anergic and suppressive following TCR engagement. However, after three rounds of restimulation, their anergic and suppressive status was abrogated.

U2 - 10.1002/1521-4141(200208)32:8<2365::AID-IMMU2365>3.0.CO;2-2

DO - 10.1002/1521-4141(200208)32:8<2365::AID-IMMU2365>3.0.CO;2-2

M3 - Article

VL - 32

SP - 2365

EP - 2375

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 8

ER -