Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

Claudio Araya; Hanna Maria Häkkinen; Luis Carcamo; Mauricio Cerda; Thierry Savy; Christopher Rookyard; Nadine Peyriéras; Jonathan D.W. Clarke

doi:10.1038/s41598-018-38455-w

Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

Claudio Araya^*, Hanna Maria Häkkinen, Luis Carcamo, Mauricio Cerda, Thierry Savy, Christopher Rookyard, Nadine Peyriéras, Jonathan D.W. Clarke

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation – the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.

Original language	English
Article number	1835
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-38455-w
Publication status	Published - 12 Feb 2019

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title = "Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate",

abstract = "Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation – the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.",

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AU - Araya, Claudio

AU - Häkkinen, Hanna Maria

AU - Carcamo, Luis

AU - Cerda, Mauricio

AU - Savy, Thierry

AU - Rookyard, Christopher

AU - Peyriéras, Nadine

AU - Clarke, Jonathan D.W.

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Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate

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