TY - JOUR
T1 - Cell- and single molecule-based methods to detect Anti-N-Methyl-D-Aspartate receptor autoantibodies in first episode psychosis patients from the OPTiMiSE project
AU - Jézéquel, Julie
AU - Rogemond, Véronique
AU - Pollak, Thomas
AU - Lepleux, Marilyn
AU - Jacobson, Leslie
AU - Gréa, Hélène
AU - Iyegbe, Conrad
AU - Kahn, Rene
AU - McGuire, Philip
AU - Vincent, Angela
AU - Honnorat, Jérôme
AU - Leboyer, Marion
AU - Groc, Laurent
PY - 2017/7/6
Y1 - 2017/7/6
N2 - Circulating autoantibodies against glutamatergic N-methyl-D-aspartate receptor (NMDAR-Ab) have been reported in a proportion of patients with psychotic disorders, raising hopes for more appropriate treatment for these antibody-positive patients. However, the prevalence of circulating NMDAR-Ab in psychotic disorders remains controversial with detection prevalence rates, and immunoglobulin (Ig) classes, varying considerably between studies, perhaps because of different detection methods. Here, we compared the results of serum assays for a large cohort of first episode psychosis patients (FEP) using classical cell-based assays in three labs and a single molecule-based imaging method. Most assays and single molecule imaging in live hippocampal neurons revealed the presence of circulating NMDAR-Ab in approximately 5% of FEP patients. However, some heterogeneity between cell-based assays was clearly observed, highlighting the urgent need of new sensitive methods to detect the presence of low-titer NMDAR-Ab in seropositive patients that cannot be clinically identified from seronegative ones.
AB - Circulating autoantibodies against glutamatergic N-methyl-D-aspartate receptor (NMDAR-Ab) have been reported in a proportion of patients with psychotic disorders, raising hopes for more appropriate treatment for these antibody-positive patients. However, the prevalence of circulating NMDAR-Ab in psychotic disorders remains controversial with detection prevalence rates, and immunoglobulin (Ig) classes, varying considerably between studies, perhaps because of different detection methods. Here, we compared the results of serum assays for a large cohort of first episode psychosis patients (FEP) using classical cell-based assays in three labs and a single molecule-based imaging method. Most assays and single molecule imaging in live hippocampal neurons revealed the presence of circulating NMDAR-Ab in approximately 5% of FEP patients. However, some heterogeneity between cell-based assays was clearly observed, highlighting the urgent need of new sensitive methods to detect the presence of low-titer NMDAR-Ab in seropositive patients that cannot be clinically identified from seronegative ones.
UR - http://www.scopus.com/inward/record.url?scp=85026768223&partnerID=8YFLogxK
U2 - 10.1016/j.biopsych.2017.06.015
DO - 10.1016/j.biopsych.2017.06.015
M3 - Article
SN - 0006-3223
JO - Biological psychiatry
JF - Biological psychiatry
ER -