Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays

Claudia Linker; Irene De Almeida; Costis Papanayotou; Matthew Stower; Virginie Sabado; Ehsan Ghorani; Andrea Streit; Roberto Mayor; Claudio D. Stern

doi:10.1016/j.ydbio.2008.12.034

Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays

Claudia Linker, Irene De Almeida, Costis Papanayotou, Matthew Stower, Virginie Sabado, Ehsan Ghorani, Andrea Streit, Roberto Mayor, Claudio D. Stern

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

In Xenopus, the animal cap is very sensitive to BMP antagonists, which result in neuralization. In chick, however, only cells at the border of the neural plate can be neuralized by BMP inhibition. Here we compare the two systems. BMP antagonists can induce neural plate border markers in both ventral Xenopus epidermis and non-neural chick epiblast. However, BMP antagonism can only neuralize ectodermal cells when the BMP-inhibited cells form a continuous trail connecting them to the neural plate or its border, suggesting that homeogenetic neuralizing factors can only travel between BMP-inhibited cells. Xenopus animal cap explants contain cells fated to contribute to the neural plate border and even to the anterior neural plate, explaining why they are so easily neuralized by BMP-inhibition. Furthermore, chick explants isolated from embryonic epiblast behave like Xenopus animal caps and express border markers. We propose that the animal cap assay in Xenopus and explant assays in the chick are unsuitable for studying instructive signals in neural induction.

Original language	English
Pages (from-to)	478 - 486
Number of pages	9
Journal	Developmental Biology
Volume	327
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2008.12.034
Publication status	Published - 15 Mar 2009

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Linker, C., De Almeida, I., Papanayotou, C., Stower, M., Sabado, V., Ghorani, E., Streit, A., Mayor, R., & Stern, C. D. (2009). Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays. Developmental Biology, 327(2), 478 - 486. https://doi.org/10.1016/j.ydbio.2008.12.034

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title = "Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays",

abstract = "In Xenopus, the animal cap is very sensitive to BMP antagonists, which result in neuralization. In chick, however, only cells at the border of the neural plate can be neuralized by BMP inhibition. Here we compare the two systems. BMP antagonists can induce neural plate border markers in both ventral Xenopus epidermis and non-neural chick epiblast. However, BMP antagonism can only neuralize ectodermal cells when the BMP-inhibited cells form a continuous trail connecting them to the neural plate or its border, suggesting that homeogenetic neuralizing factors can only travel between BMP-inhibited cells. Xenopus animal cap explants contain cells fated to contribute to the neural plate border and even to the anterior neural plate, explaining why they are so easily neuralized by BMP-inhibition. Furthermore, chick explants isolated from embryonic epiblast behave like Xenopus animal caps and express border markers. We propose that the animal cap assay in Xenopus and explant assays in the chick are unsuitable for studying instructive signals in neural induction.",

author = "Claudia Linker and {De Almeida}, Irene and Costis Papanayotou and Matthew Stower and Virginie Sabado and Ehsan Ghorani and Andrea Streit and Roberto Mayor and Stern, {Claudio D.}",

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Linker, C, De Almeida, I, Papanayotou, C, Stower, M, Sabado, V, Ghorani, E, Streit, A, Mayor, R & Stern, CD 2009, 'Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays', Developmental Biology, vol. 327, no. 2, pp. 478 - 486. https://doi.org/10.1016/j.ydbio.2008.12.034

Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays. / Linker, Claudia; De Almeida, Irene; Papanayotou, Costis et al.
In: Developmental Biology, Vol. 327, No. 2, 15.03.2009, p. 478 - 486.

Research output: Contribution to journal › Article › peer-review

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AU - Streit, Andrea

AU - Mayor, Roberto

AU - Stern, Claudio D.

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