Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery

Arnie Purushotham; Aaditya Sinha; zhane paterson; belul shifa; hannah jeffrey; Patriek Jurrius; Sarah Allen; Eugene Lee; Mohammed Azmat; Rachel Barrass; Damion Bailey; Jessica Johnson; Vasileios  Karydakis; Kathryn Adamson; elina shaari; Mangesh A Thorat; Hisham Hamed; Georgina Bitsakou; Sarah Pinder; Padma Menon; Wen Ng; Gary Cook; Joemon John; Armidita Jacob; Sofia Pereira; Jocelyn Thomas; Ruheana Begum; Kariem El-Boghdadly; Mieke Van Hemelrijck; Ashutosh Kothari

Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery

Arnie Purushotham^*, Aaditya Sinha, zhane paterson, belul shifa, hannah jeffrey, Patriek Jurrius, Sarah Allen, Eugene Lee, Mohammed Azmat, Rachel Barrass, Damion Bailey, Jessica Johnson, Vasileios Karydakis, Kathryn Adamson, elina shaari, Mangesh A Thorat, Hisham Hamed, Georgina Bitsakou, Sarah Pinder, Padma MenonWen Ng, Gary Cook, Joemon John, Armidita Jacob, Sofia Pereira, Jocelyn Thomas, Ruheana Begum, Kariem El-Boghdadly, Mieke Van Hemelrijck, Ashutosh Kothari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Background
Among women with breast cancer who undergo breast-conserving surgery (BCS), 20-25% require further surgery due to close or involved margins. Improved techniques are needed to assess resection margins.

Purpose
The study aims were to assess the feasibility of the combined techniques of Cerenkov Luminescence Imaging - Flexible AutoRadiography (CLI-FAR) to assess excision specimen margins in women undergoing BCS and to determine the diagnostic performance of intraoperative CLI-FAR imaging with postoperative histopathology as the reference standard.
.
Materials and Methods
Women undergoing BCS were recruited prospectively at a single centre over thirteen months. Patients were injected with 250MBq +/- 10MBq of 18F-fluorodeoxyglucose (18F-FDG), 145 minutes before surgery and the excised specimens were imaged intraoperatively. The surgically excised tumour was initially imaged using conventional x-ray, and margins suspected to be involved by tumor were then imaged using CLI-FAR. CLI-FAR imaging was performed using the LightPath system® (Lightpoint®), an in vitro diagnostic device designed to identify and locate positron-emitting radionuclides. Any suspicious margin underwent an immediate re-excision in the form of cavity shavings. Sensitivity, specificity, positive and negative predictive value whilst considering histopathological assessment as the golden standard were used to assess the performance of CLI-FAR.

Results
In all, 54 specimens were imaged in 52 patients with a total of 104 margins reviewed using CLI-FAR. The results showed a specificity of 97.8% (89/91, 95% CI: 95.0%, 100.6%), sensitivity of 76.9% (10/13, 95% CI: 68.3%, 85.0%), PPV of 83.3% (10/12, 95% CI: 76.2%, 90.5%) and NPV of 96.7% (89/92, 95% CI: 93.3%, 100.2%). In all, 8 patients had 10 positive margins on CLI-FAR imaging and were treated accordingly. CLI-FAR imaging reduced the re-excision rate by (17.3/25) 69%.

Conclusion
CLI-FAR imaging is a promising technique for intraoperative margin assessment in women undergoing BCS for invasive breast cancer.

Original language	English
Journal	Radiology Advances
Publication status	Accepted/In press - 16 May 2024

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Cerenkov Luminescence Imaging and_Purushotham_Accepted16May2024_GREEN AAMAccepted author manuscript, 441 KBLicence: CC BY

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Purushotham, A., Sinha, A., paterson, Z., shifa, B., jeffrey, H., Jurrius, P., Allen, S., Lee, E., Azmat, M., Barrass, R., Bailey, D., Johnson, J., Karydakis, V., Adamson, K., shaari, E., Thorat, M. A., Hamed, H., Bitsakou, G., Pinder, S., ... Kothari, A. (Accepted/In press). Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery. Radiology Advances.

@article{0a645f4658c64ba9ba0eab6c92514057,

title = "Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery",

abstract = "Background Among women with breast cancer who undergo breast-conserving surgery (BCS), 20-25% require further surgery due to close or involved margins. Improved techniques are needed to assess resection margins.PurposeThe study aims were to assess the feasibility of the combined techniques of Cerenkov Luminescence Imaging - Flexible AutoRadiography (CLI-FAR) to assess excision specimen margins in women undergoing BCS and to determine the diagnostic performance of intraoperative CLI-FAR imaging with postoperative histopathology as the reference standard..Materials and Methods Women undergoing BCS were recruited prospectively at a single centre over thirteen months. Patients were injected with 250MBq +/- 10MBq of 18F-fluorodeoxyglucose (18F-FDG), 145 minutes before surgery and the excised specimens were imaged intraoperatively. The surgically excised tumour was initially imaged using conventional x-ray, and margins suspected to be involved by tumor were then imaged using CLI-FAR. CLI-FAR imaging was performed using the LightPath system{\textregistered} (Lightpoint{\textregistered}), an in vitro diagnostic device designed to identify and locate positron-emitting radionuclides. Any suspicious margin underwent an immediate re-excision in the form of cavity shavings. Sensitivity, specificity, positive and negative predictive value whilst considering histopathological assessment as the golden standard were used to assess the performance of CLI-FAR.ResultsIn all, 54 specimens were imaged in 52 patients with a total of 104 margins reviewed using CLI-FAR. The results showed a specificity of 97.8% (89/91, 95% CI: 95.0%, 100.6%), sensitivity of 76.9% (10/13, 95% CI: 68.3%, 85.0%), PPV of 83.3% (10/12, 95% CI: 76.2%, 90.5%) and NPV of 96.7% (89/92, 95% CI: 93.3%, 100.2%). In all, 8 patients had 10 positive margins on CLI-FAR imaging and were treated accordingly. CLI-FAR imaging reduced the re-excision rate by (17.3/25) 69%. ConclusionCLI-FAR imaging is a promising technique for intraoperative margin assessment in women undergoing BCS for invasive breast cancer. ",

author = "Arnie Purushotham and Aaditya Sinha and zhane paterson and belul shifa and hannah jeffrey and Patriek Jurrius and Sarah Allen and Eugene Lee and Mohammed Azmat and Rachel Barrass and Damion Bailey and Jessica Johnson and Vasileios Karydakis and Kathryn Adamson and elina shaari and Thorat, {Mangesh A} and Hisham Hamed and Georgina Bitsakou and Sarah Pinder and Padma Menon and Wen Ng and Gary Cook and Joemon John and Armidita Jacob and Sofia Pereira and Jocelyn Thomas and Ruheana Begum and Kariem El-Boghdadly and {Van Hemelrijck}, Mieke and Ashutosh Kothari",

year = "2024",

month = may,

day = "16",

language = "English",

journal = "Radiology Advances",

}

Purushotham, A , Sinha, A, paterson, Z, shifa, B, jeffrey, H, Jurrius, P , Allen, S, Lee, E, Azmat, M, Barrass, R, Bailey, D, Johnson, J, Karydakis, V, Adamson, K, shaari, E, Thorat, MA, Hamed, H, Bitsakou, G, Pinder, S, Menon, P, Ng, W, Cook, G, John, J, Jacob, A, Pereira, S, Thomas, J, Begum, R, El-Boghdadly, K , Van Hemelrijck, M & Kothari, A 2024, 'Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery', Radiology Advances.

TY - JOUR

T1 - Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery

AU - Purushotham, Arnie

AU - Sinha, Aaditya

AU - paterson, zhane

AU - shifa, belul

AU - jeffrey, hannah

AU - Jurrius, Patriek

AU - Allen, Sarah

AU - Lee, Eugene

AU - Azmat, Mohammed

AU - Barrass, Rachel

AU - Bailey, Damion

AU - Johnson, Jessica

AU - Karydakis, Vasileios

AU - Adamson, Kathryn

AU - shaari, elina

AU - Thorat, Mangesh A

AU - Hamed, Hisham

AU - Bitsakou, Georgina

AU - Pinder, Sarah

AU - Menon, Padma

AU - Ng, Wen

AU - Cook, Gary

AU - John, Joemon

AU - Jacob, Armidita

AU - Pereira, Sofia

AU - Thomas, Jocelyn

AU - Begum, Ruheana

AU - El-Boghdadly, Kariem

AU - Van Hemelrijck, Mieke

AU - Kothari, Ashutosh

PY - 2024/5/16

Y1 - 2024/5/16

N2 - Background Among women with breast cancer who undergo breast-conserving surgery (BCS), 20-25% require further surgery due to close or involved margins. Improved techniques are needed to assess resection margins.PurposeThe study aims were to assess the feasibility of the combined techniques of Cerenkov Luminescence Imaging - Flexible AutoRadiography (CLI-FAR) to assess excision specimen margins in women undergoing BCS and to determine the diagnostic performance of intraoperative CLI-FAR imaging with postoperative histopathology as the reference standard..Materials and Methods Women undergoing BCS were recruited prospectively at a single centre over thirteen months. Patients were injected with 250MBq +/- 10MBq of 18F-fluorodeoxyglucose (18F-FDG), 145 minutes before surgery and the excised specimens were imaged intraoperatively. The surgically excised tumour was initially imaged using conventional x-ray, and margins suspected to be involved by tumor were then imaged using CLI-FAR. CLI-FAR imaging was performed using the LightPath system® (Lightpoint®), an in vitro diagnostic device designed to identify and locate positron-emitting radionuclides. Any suspicious margin underwent an immediate re-excision in the form of cavity shavings. Sensitivity, specificity, positive and negative predictive value whilst considering histopathological assessment as the golden standard were used to assess the performance of CLI-FAR.ResultsIn all, 54 specimens were imaged in 52 patients with a total of 104 margins reviewed using CLI-FAR. The results showed a specificity of 97.8% (89/91, 95% CI: 95.0%, 100.6%), sensitivity of 76.9% (10/13, 95% CI: 68.3%, 85.0%), PPV of 83.3% (10/12, 95% CI: 76.2%, 90.5%) and NPV of 96.7% (89/92, 95% CI: 93.3%, 100.2%). In all, 8 patients had 10 positive margins on CLI-FAR imaging and were treated accordingly. CLI-FAR imaging reduced the re-excision rate by (17.3/25) 69%. ConclusionCLI-FAR imaging is a promising technique for intraoperative margin assessment in women undergoing BCS for invasive breast cancer.

AB - Background Among women with breast cancer who undergo breast-conserving surgery (BCS), 20-25% require further surgery due to close or involved margins. Improved techniques are needed to assess resection margins.PurposeThe study aims were to assess the feasibility of the combined techniques of Cerenkov Luminescence Imaging - Flexible AutoRadiography (CLI-FAR) to assess excision specimen margins in women undergoing BCS and to determine the diagnostic performance of intraoperative CLI-FAR imaging with postoperative histopathology as the reference standard..Materials and Methods Women undergoing BCS were recruited prospectively at a single centre over thirteen months. Patients were injected with 250MBq +/- 10MBq of 18F-fluorodeoxyglucose (18F-FDG), 145 minutes before surgery and the excised specimens were imaged intraoperatively. The surgically excised tumour was initially imaged using conventional x-ray, and margins suspected to be involved by tumor were then imaged using CLI-FAR. CLI-FAR imaging was performed using the LightPath system® (Lightpoint®), an in vitro diagnostic device designed to identify and locate positron-emitting radionuclides. Any suspicious margin underwent an immediate re-excision in the form of cavity shavings. Sensitivity, specificity, positive and negative predictive value whilst considering histopathological assessment as the golden standard were used to assess the performance of CLI-FAR.ResultsIn all, 54 specimens were imaged in 52 patients with a total of 104 margins reviewed using CLI-FAR. The results showed a specificity of 97.8% (89/91, 95% CI: 95.0%, 100.6%), sensitivity of 76.9% (10/13, 95% CI: 68.3%, 85.0%), PPV of 83.3% (10/12, 95% CI: 76.2%, 90.5%) and NPV of 96.7% (89/92, 95% CI: 93.3%, 100.2%). In all, 8 patients had 10 positive margins on CLI-FAR imaging and were treated accordingly. CLI-FAR imaging reduced the re-excision rate by (17.3/25) 69%. ConclusionCLI-FAR imaging is a promising technique for intraoperative margin assessment in women undergoing BCS for invasive breast cancer.

M3 - Article

JO - Radiology Advances

JF - Radiology Advances

ER -

Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery

Abstract

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