Cerenkov Luminescence Imaging and Flexible Autoradiography for specimen margin assessment during breast-conserving cancer surgery

Arnie Purushotham*, Aaditya Sinha, zhane paterson, belul shifa, hannah jeffrey, Patriek Jurrius, Sarah Allen, Eugene Lee, Mohammed Azmat, Rachel Barrass, Damion Bailey, Jessica Johnson, Vasileios Karydakis, Kathryn Adamson, elina shaari, Mangesh A Thorat, Hisham Hamed, Georgina Bitsakou, Sarah Pinder, Padma MenonWen Ng, Gary Cook, Joemon John, Armidita Jacob, Sofia Pereira, Jocelyn Thomas, Ruheana Begum, Kariem El-Boghdadly, Mieke Van Hemelrijck, Ashutosh Kothari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Among women with breast cancer who undergo breast-conserving surgery (BCS), 20-25% require further surgery due to close or involved margins. Improved techniques are needed to assess resection margins.

The study aims were to assess the feasibility of the combined techniques of Cerenkov Luminescence Imaging - Flexible AutoRadiography (CLI-FAR) to assess excision specimen margins in women undergoing BCS and to determine the diagnostic performance of intraoperative CLI-FAR imaging with postoperative histopathology as the reference standard.
Materials and Methods
Women undergoing BCS were recruited prospectively at a single centre over thirteen months. Patients were injected with 250MBq +/- 10MBq of 18F-fluorodeoxyglucose (18F-FDG), 145 minutes before surgery and the excised specimens were imaged intraoperatively. The surgically excised tumour was initially imaged using conventional x-ray, and margins suspected to be involved by tumor were then imaged using CLI-FAR. CLI-FAR imaging was performed using the LightPath system® (Lightpoint®), an in vitro diagnostic device designed to identify and locate positron-emitting radionuclides. Any suspicious margin underwent an immediate re-excision in the form of cavity shavings. Sensitivity, specificity, positive and negative predictive value whilst considering histopathological assessment as the golden standard were used to assess the performance of CLI-FAR.

In all, 54 specimens were imaged in 52 patients with a total of 104 margins reviewed using CLI-FAR. The results showed a specificity of 97.8% (89/91, 95% CI: 95.0%, 100.6%), sensitivity of 76.9% (10/13, 95% CI: 68.3%, 85.0%), PPV of 83.3% (10/12, 95% CI: 76.2%, 90.5%) and NPV of 96.7% (89/92, 95% CI: 93.3%, 100.2%). In all, 8 patients had 10 positive margins on CLI-FAR imaging and were treated accordingly. CLI-FAR imaging reduced the re-excision rate by (17.3/25) 69%.

CLI-FAR imaging is a promising technique for intraoperative margin assessment in women undergoing BCS for invasive breast cancer.
Original languageEnglish
JournalRadiology Advances
Publication statusAccepted/In press - 16 May 2024


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