Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study

Kate M. Bermingham, Mohsen Mazidi, Paul w. Franks, Tyler Maher, Ana m. Valdes, Inbar Linenberg, Jonathan Wolf, George Hadjigeorgiou, Tim d. Spector, Cristina Menni, Jose m. Ordovas, Sarah e. Berry, Wendy l. Hall

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDICT 1 cohort. Methods: In the ZOE PREDICT 1 study (n = 1002 (NCT03479866)), 250 metabolites, mainly lipids, were measured by a Nightingale NMR panel in fasting and postprandial (4 and 6 h after a 3.7 MJ mixed nutrient meal, with a second 2.2 MJ mixed nutrient meal at 4 h) serum samples. For each metabolite, inter- and intra-individual variability over time was evaluated using linear mixed modelling and intraclass correlation coefficients (ICC) were calculated. Results: Postprandially, 85% (of 250 metabolites) significantly changed from fasting at 6 h (47% increased, 53% decreased; Kruskal–Wallis), with 37 measures increasing by >25% and 14 increasing by >50%. The largest changes were observed in very large lipoprotein particles and ketone bodies. Seventy-one percent of circulating metabolites were strongly correlated (Spearman’s rho >0.80) between fasting and postprandial timepoints, and 5% were weakly correlated (rho
Original languageEnglish
Article number2638
Issue number11
Early online date5 Jun 2023
Publication statusPublished - Jun 2023


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