Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study

TY - JOUR

T1 - Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study

AU - Bermingham, Kate M.

AU - Mazidi, Mohsen

AU - Franks, Paul w.

AU - Maher, Tyler

AU - Valdes, Ana m.

AU - Linenberg, Inbar

AU - Wolf, Jonathan

AU - Hadjigeorgiou, George

AU - Spector, Tim d.

AU - Menni, Cristina

AU - Ordovas, Jose m.

AU - Berry, Sarah e.

AU - Hall, Wendy l.

N1 - Funding Information: This work was supported by ZOE Ltd. and TwinsUK, which are funded by the Wellcome Trust, Medical Research Council, Versus Arthritis, European Union Horizon 2020, Chronic Disease Research Foundation (CDRF), ZOE Ltd. and the National Institute for Health Research (NIHR), Clinical Research Network (CRN) and Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust in partnership with King’s College London. The study sponsors (ZOE Ltd.) contributed as part of the Scientific Advisory Board in the study design and collection. CM is funded by the Chronic Disease Research Foundation. The study sponsors (ZOE Ltd.) contributed as part of the Scientific Advisory Board in the study design and collection. Publisher Copyright: © 2023 by the authors.

PY - 2023/6

Y1 - 2023/6

N2 - Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDICT 1 cohort. Methods: In the ZOE PREDICT 1 study (n = 1002 (NCT03479866)), 250 metabolites, mainly lipids, were measured by a Nightingale NMR panel in fasting and postprandial (4 and 6 h after a 3.7 MJ mixed nutrient meal, with a second 2.2 MJ mixed nutrient meal at 4 h) serum samples. For each metabolite, inter- and intra-individual variability over time was evaluated using linear mixed modelling and intraclass correlation coefficients (ICC) were calculated. Results: Postprandially, 85% (of 250 metabolites) significantly changed from fasting at 6 h (47% increased, 53% decreased; Kruskal–Wallis), with 37 measures increasing by >25% and 14 increasing by >50%. The largest changes were observed in very large lipoprotein particles and ketone bodies. Seventy-one percent of circulating metabolites were strongly correlated (Spearman’s rho >0.80) between fasting and postprandial timepoints, and 5% were weakly correlated (rho

AB - Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDICT 1 cohort. Methods: In the ZOE PREDICT 1 study (n = 1002 (NCT03479866)), 250 metabolites, mainly lipids, were measured by a Nightingale NMR panel in fasting and postprandial (4 and 6 h after a 3.7 MJ mixed nutrient meal, with a second 2.2 MJ mixed nutrient meal at 4 h) serum samples. For each metabolite, inter- and intra-individual variability over time was evaluated using linear mixed modelling and intraclass correlation coefficients (ICC) were calculated. Results: Postprandially, 85% (of 250 metabolites) significantly changed from fasting at 6 h (47% increased, 53% decreased; Kruskal–Wallis), with 37 measures increasing by >25% and 14 increasing by >50%. The largest changes were observed in very large lipoprotein particles and ketone bodies. Seventy-one percent of circulating metabolites were strongly correlated (Spearman’s rho >0.80) between fasting and postprandial timepoints, and 5% were weakly correlated (rho

UR - http://www.scopus.com/inward/record.url?scp=85161382348&partnerID=8YFLogxK

U2 - 10.3390/nu15112638

DO - 10.3390/nu15112638

M3 - Article

SN - 2072-6643

VL - 15

JO - Nutrients

JF - Nutrients

IS - 11

M1 - 2638

ER -