Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants

Lesley A  Turner; Aleksandar Vjestica; Alaina H  Willet; Snezhana Oliferenko; Kathleen L Gould

doi:10.17912/micropub.biology.001515

Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants

Lesley A Turner, Aleksandar Vjestica, Alaina H Willet, Snezhana Oliferenko, Kathleen L Gould

Research output: Contribution to journal › Article › peer-review

Abstract

The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast Schizosaccharomyces pombe , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared cdc15 temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each cdc15 mutant allele and analyzed their growth at different temperatures. Additionally, we report a new cdc15 allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in S. pombe.

Original language	English
Journal	microPublication Biology
DOIs	https://doi.org/10.17912/micropub.biology.001515
Publication status	Published - 25 Mar 2025

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10.17912/micropub.biology.001515Licence: CC BY

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title = "Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants",

abstract = "The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast Schizosaccharomyces pombe , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared cdc15 temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each cdc15 mutant allele and analyzed their growth at different temperatures. Additionally, we report a new cdc15 allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in S. pombe.",

author = "Turner, {Lesley A} and Aleksandar Vjestica and Willet, {Alaina H} and Snezhana Oliferenko and Gould, {Kathleen L}",

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doi = "10.17912/micropub.biology.001515",

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AU - Turner, Lesley A

AU - Vjestica, Aleksandar

AU - Willet, Alaina H

AU - Oliferenko, Snezhana

AU - Gould, Kathleen L

PY - 2025/3/25

Y1 - 2025/3/25

N2 - The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast Schizosaccharomyces pombe , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared cdc15 temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each cdc15 mutant allele and analyzed their growth at different temperatures. Additionally, we report a new cdc15 allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in S. pombe.

AB - The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast Schizosaccharomyces pombe , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared cdc15 temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each cdc15 mutant allele and analyzed their growth at different temperatures. Additionally, we report a new cdc15 allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in S. pombe.

U2 - 10.17912/micropub.biology.001515

DO - 10.17912/micropub.biology.001515

M3 - Article

SN - 2578-9430

JO - microPublication Biology

JF - microPublication Biology

ER -

Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants

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