TY - JOUR
T1 - Characterization and Validation of Radiotracer Kinetics Using the Langendorff Isolated Perfused Heart
AU - Baark, Friedrich
AU - Waters, Edward C T
AU - Eykyn, Thomas R
AU - Southworth, Richard
N1 - Funding Information:
NIHR Biomedical Research Centre at Guy’s and St Thomas’ NHS Foundation Trust and KCL; the Centre of Excellence in Medical Engineering funded by the Wellcome Trust and Engineering and EPSRC (WT 203148/Z/16/Z); the BHF Centre of Research Excellence (RE/18/2/34213) and the KCL Comprehensive Can-cer Imaging Centre funded by the Cancer Research UK and EPSRC in association with the MRC and the Department of health (DoH). British Heart Foundation [PG/15/60/31629] and [PG/16/43/32141]; EPSRC Programs EP/S032789/1 and EP/S019901/1 [EP/M506357/1]; [SR1] Wellcome/EPSRC Centre for Medical Engineering [WT 203148/Z/16/Z]; National Institute for Health Research (NIHR[SR2]). The views expressed are those of the authors and not necessarily those of the EPSRC, BHF, NHS, the NIHR, or DoH.
Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - The Langendorff isolated perfused heart is a physiologically relevant and controllable ex vivo model well suited to characterizing and validating novel radiotracers for a wide range of molecular imaging applications. It allows the monitoring of first pass tracer uptake kinetics either as a bolus injection or as a continuous infusion in beating myocardial tissue with a high degree of experimental control in terms of cardiac workload, perfusion, energy substrate delivery and composition, and drug co-administration. The radiotracer pharmacokinetic data that it provides is not contaminated by confounding factors such as off-target tracer metabolism, and as a non-imaging technique, time activity curves can be acquired with very high temporal resolution. In this chapter, we describe the basic principles and practice for setting up and using Langendorff isolated perfused hearts for the assessment of novel radiotracers and outline their potential for modeling pathophysiological conditions relevant to cardiovascular disease.
AB - The Langendorff isolated perfused heart is a physiologically relevant and controllable ex vivo model well suited to characterizing and validating novel radiotracers for a wide range of molecular imaging applications. It allows the monitoring of first pass tracer uptake kinetics either as a bolus injection or as a continuous infusion in beating myocardial tissue with a high degree of experimental control in terms of cardiac workload, perfusion, energy substrate delivery and composition, and drug co-administration. The radiotracer pharmacokinetic data that it provides is not contaminated by confounding factors such as off-target tracer metabolism, and as a non-imaging technique, time activity curves can be acquired with very high temporal resolution. In this chapter, we describe the basic principles and practice for setting up and using Langendorff isolated perfused hearts for the assessment of novel radiotracers and outline their potential for modeling pathophysiological conditions relevant to cardiovascular disease.
KW - Heart/diagnostic imaging
KW - Myocardium/metabolism
KW - Radiopharmaceuticals/metabolism
KW - Kinetics
KW - Perfusion/methods
UR - http://www.scopus.com/inward/record.url?scp=85177789087&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3499-8_15
DO - 10.1007/978-1-0716-3499-8_15
M3 - Article
C2 - 38006501
SN - 1064-3745
VL - 2729
SP - 251
EP - 267
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -
