Characterization of gastric mucosa biopsies reveals alterations in huntington's disease

Andrew C. McCourt; Kirsty L. O'donovan; Eva Ekblad; Elin Sand; David Craufurd; Anne Rosser; David Sanders; Nicholas Stoy; Hugh Rickards; Nils Wierup; Gillian P. Bates; Maria Björkqvist; Oliver Quarrell

doi:10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

Characterization of gastric mucosa biopsies reveals alterations in huntington's disease

Andrew C. McCourt, Kirsty L. O'donovan, Eva Ekblad, Elin Sand, David Craufurd, Anne Rosser, David Sanders, Nicholas Stoy, Hugh Rickards^*, Nils Wierup, Gillian P. Bates, Maria Björkqvist, Oliver Quarrell

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Weight loss is an important complication of Huntington’s disease (HD), however the mechanism for weight loss in HD is not entirely understood. Mutant huntingtin is expressed in the gastrointestinal (GI) tract and, in HD mice, mutant huntingtin inclusions are found within the enteric nervous system along the GI tract. A reduction of neuropeptides, decreased mucosal thickness and villus length, as well as gut motility impairment, have also been shown in HD mice. We therefore set out to study gastric mucosa of patients with HD, looking for abnormalities of mucosal cells using immunohistochemistry. In order to investigate possible histological differences related to gastric acid production, we evaluated the cell density of acid producing parietal cells, as well as gastrin producing cells (the endocrine cell controlling parietal cell function). In addition, we looked at chief cells and somatostatin-containing cells. In gastric mucosa from HD subjects, compared to control subject biopsies, a reduced expression of gastrin (a marker of G cells) was found. This is in line with previous HD mouse studies showing reduction of GI tract neuropeptides.

Original language	English
Journal	PLOS Currents: Disasters
Volume	7
Issue number	HUNTINGTONDISEASE
DOIs	https://doi.org/10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79
Publication status	Published - 26 Jun 2015

Access to Document

10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

Cite this

McCourt, A. C., O'donovan, K. L., Ekblad, E., Sand, E., Craufurd, D., Rosser, A., Sanders, D., Stoy, N., Rickards, H., Wierup, N., Bates, G. P., Björkqvist, M., & Quarrell, O. (2015). Characterization of gastric mucosa biopsies reveals alterations in huntington's disease. PLOS Currents: Disasters, 7(HUNTINGTONDISEASE). https://doi.org/10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

@article{0c41337119cb498fae66dd4cf2b5ef38,

title = "Characterization of gastric mucosa biopsies reveals alterations in huntington's disease",

abstract = "Weight loss is an important complication of Huntington{\textquoteright}s disease (HD), however the mechanism for weight loss in HD is not entirely understood. Mutant huntingtin is expressed in the gastrointestinal (GI) tract and, in HD mice, mutant huntingtin inclusions are found within the enteric nervous system along the GI tract. A reduction of neuropeptides, decreased mucosal thickness and villus length, as well as gut motility impairment, have also been shown in HD mice. We therefore set out to study gastric mucosa of patients with HD, looking for abnormalities of mucosal cells using immunohistochemistry. In order to investigate possible histological differences related to gastric acid production, we evaluated the cell density of acid producing parietal cells, as well as gastrin producing cells (the endocrine cell controlling parietal cell function). In addition, we looked at chief cells and somatostatin-containing cells. In gastric mucosa from HD subjects, compared to control subject biopsies, a reduced expression of gastrin (a marker of G cells) was found. This is in line with previous HD mouse studies showing reduction of GI tract neuropeptides.",

author = "McCourt, {Andrew C.} and O'donovan, {Kirsty L.} and Eva Ekblad and Elin Sand and David Craufurd and Anne Rosser and David Sanders and Nicholas Stoy and Hugh Rickards and Nils Wierup and Bates, {Gillian P.} and Maria Bj{\"o}rkqvist and Oliver Quarrell",

year = "2015",

month = jun,

day = "26",

doi = "10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79",

language = "English",

volume = "7",

journal = "PLOS Currents: Disasters",

issn = "2157-3999",

number = "HUNTINGTONDISEASE",

}

McCourt, AC, O'donovan, KL, Ekblad, E, Sand, E, Craufurd, D, Rosser, A, Sanders, D, Stoy, N, Rickards, H, Wierup, N, Bates, GP, Björkqvist, M & Quarrell, O 2015, 'Characterization of gastric mucosa biopsies reveals alterations in huntington's disease', PLOS Currents: Disasters, vol. 7, no. HUNTINGTONDISEASE. https://doi.org/10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

TY - JOUR

T1 - Characterization of gastric mucosa biopsies reveals alterations in huntington's disease

AU - McCourt, Andrew C.

AU - O'donovan, Kirsty L.

AU - Ekblad, Eva

AU - Sand, Elin

AU - Craufurd, David

AU - Rosser, Anne

AU - Sanders, David

AU - Stoy, Nicholas

AU - Rickards, Hugh

AU - Wierup, Nils

AU - Bates, Gillian P.

AU - Björkqvist, Maria

AU - Quarrell, Oliver

PY - 2015/6/26

Y1 - 2015/6/26

N2 - Weight loss is an important complication of Huntington’s disease (HD), however the mechanism for weight loss in HD is not entirely understood. Mutant huntingtin is expressed in the gastrointestinal (GI) tract and, in HD mice, mutant huntingtin inclusions are found within the enteric nervous system along the GI tract. A reduction of neuropeptides, decreased mucosal thickness and villus length, as well as gut motility impairment, have also been shown in HD mice. We therefore set out to study gastric mucosa of patients with HD, looking for abnormalities of mucosal cells using immunohistochemistry. In order to investigate possible histological differences related to gastric acid production, we evaluated the cell density of acid producing parietal cells, as well as gastrin producing cells (the endocrine cell controlling parietal cell function). In addition, we looked at chief cells and somatostatin-containing cells. In gastric mucosa from HD subjects, compared to control subject biopsies, a reduced expression of gastrin (a marker of G cells) was found. This is in line with previous HD mouse studies showing reduction of GI tract neuropeptides.

AB - Weight loss is an important complication of Huntington’s disease (HD), however the mechanism for weight loss in HD is not entirely understood. Mutant huntingtin is expressed in the gastrointestinal (GI) tract and, in HD mice, mutant huntingtin inclusions are found within the enteric nervous system along the GI tract. A reduction of neuropeptides, decreased mucosal thickness and villus length, as well as gut motility impairment, have also been shown in HD mice. We therefore set out to study gastric mucosa of patients with HD, looking for abnormalities of mucosal cells using immunohistochemistry. In order to investigate possible histological differences related to gastric acid production, we evaluated the cell density of acid producing parietal cells, as well as gastrin producing cells (the endocrine cell controlling parietal cell function). In addition, we looked at chief cells and somatostatin-containing cells. In gastric mucosa from HD subjects, compared to control subject biopsies, a reduced expression of gastrin (a marker of G cells) was found. This is in line with previous HD mouse studies showing reduction of GI tract neuropeptides.

UR - http://www.scopus.com/inward/record.url?scp=84958580999&partnerID=8YFLogxK

U2 - 10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

DO - 10.1371/currents.hd.858b4cc7f235df068387e9c20c436a79

M3 - Article

AN - SCOPUS:84958580999

SN - 2157-3999

VL - 7

JO - PLOS Currents: Disasters

JF - PLOS Currents: Disasters

IS - HUNTINGTONDISEASE

ER -

Characterization of gastric mucosa biopsies reveals alterations in huntington's disease

Abstract

Access to Document

Other files and links

Fingerprint

Cite this