Characterization of glioblastoma in an orthotopic mouse model with magnetic resonance elastography

Katharina Schregel*, Navid Nazari, Michal O. Nowicki, Miklos Palotai, Sean E. Lawler, Ralph Sinkus, Paul E. Barbone, Samuel Patz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Glioblastoma (GBM) is the most common primary brain tumor. It is highly malignant and has a correspondingly poor prognosis. Diagnosis and monitoring are mainly accomplished with MRI, but remain challenging in some cases. Therefore, complementary methods for tumor detection and characterization would be beneficial. Using magnetic resonance elastography (MRE), we performed a longitudinal study of the biomechanical properties of intracranially implanted GBM in mice and compared the results to histopathology. The biomechanical parameters of viscoelastic modulus, shear wave speed and phase angle were significantly lower in tumors compared with healthy brain tissue and decreased over time with tumor progression. Moreover, some MRE parameters revealed sub-regions at later tumor stages, which were not easily detectable on anatomical MRI images. Comparison with histopathology showed that softer tumor regions contained necrosis and patches of viable tumor cells. In contrast, areas of densely packed tumor cells and blood vessels identified with histology coincided with higher values of viscoelastic modulus and shear wave speed. Interestingly, the phase angle was independent from these anatomical variations. In summary, MRE depicted longitudinal and morphological changes in GBM and may prove valuable for tumor characterization in patients.

Original languageEnglish
JournalNMR in Biomedicine
DOIs
Publication statusAccepted/In press - 1 Jan 2017

Keywords

  • Brain tumor
  • Glioblastoma
  • Longitudinal progression
  • Magnetic resonance elastography
  • Stiffness
  • Viscoelastic modulus

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