Characterization of the mouse adeno-associated virus AAVS1 ortholog

TY - JOUR

T1 - Characterization of the mouse adeno-associated virus AAVS1 ortholog

AU - Dutheil, Nathalie

AU - Yoon-Robarts, Miran

AU - Ward, Peter

AU - Henckaerts, Els

AU - Skrabanek, Lucy

AU - Berns, Kenneth I

AU - Campagne, Fabien

AU - Linden, R Michael

PY - 2004/8

Y1 - 2004/8

N2 - The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proximal to the recently identified Mbs85 gene. These sequence motifs can be specifically nicked by the viral Rep protein required for the initiation of site-specific AAV DNA integration. Furthermore, these signals can serve as a minimal origin for Rep-dependent DNA replication. In addition, we isolated the mouse Mbs85 proximal promoter and show transcriptional activity in three mouse cell lines.

AB - The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proximal to the recently identified Mbs85 gene. These sequence motifs can be specifically nicked by the viral Rep protein required for the initiation of site-specific AAV DNA integration. Furthermore, these signals can serve as a minimal origin for Rep-dependent DNA replication. In addition, we isolated the mouse Mbs85 proximal promoter and show transcriptional activity in three mouse cell lines.

U2 - 10.1128/JVI.78.16.8917-8921.2004

DO - 10.1128/JVI.78.16.8917-8921.2004

M3 - Article

C2 - 15280500

SN - 1098-5514

VL - 78

SP - 8917

EP - 8921

JO - Journal of Virology

JF - Journal of Virology

IS - 16

ER -