Chemical composition of and inhibition of angiotensin-converting enzyme by Senecio samnitum huet

R Tundis; M R Loizzo; G A Statti; B Deguin; R Amissah; P J Houghton; F Menichini

doi:10.1080/13880200500301886

Chemical composition of and inhibition of angiotensin-converting enzyme by Senecio samnitum huet

R Tundis, M R Loizzo, G A Statti, B Deguin, R Amissah, P J Houghton, F Menichini

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Abstract

Extracts of Senecio samnitum Huet and derived methyl ester of chlorogenic acid have been shown to inhibit angiotensin-converting enzyme (ACE) by using an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labeled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by high-performance liquid chromatography (HPLC). GC/MS and NMR identified compounds present within the studied S. samnitum extracts. The most effective fraction was obtained in ethyl acetate, which gave 52.56 +/- 0.23% (SD) inhibition at 300 mu g/ml. The major constituent of this fraction, the methyl ester of chlorogenic acid, showed significant ACE inhibition of 56.78 +/- 0.25% at a concentration of 82.5 mu g/ml

Original language	English
Pages (from-to)	605 - 608
Number of pages	4
Journal	PHARMACEUTICAL BIOLOGY
Volume	43
Issue number	7
DOIs	https://doi.org/10.1080/13880200500301886
Publication status	Published - Oct 2005

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title = "Chemical composition of and inhibition of angiotensin-converting enzyme by Senecio samnitum huet",

abstract = "Extracts of Senecio samnitum Huet and derived methyl ester of chlorogenic acid have been shown to inhibit angiotensin-converting enzyme (ACE) by using an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labeled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by high-performance liquid chromatography (HPLC). GC/MS and NMR identified compounds present within the studied S. samnitum extracts. The most effective fraction was obtained in ethyl acetate, which gave 52.56 +/- 0.23% (SD) inhibition at 300 mu g/ml. The major constituent of this fraction, the methyl ester of chlorogenic acid, showed significant ACE inhibition of 56.78 +/- 0.25% at a concentration of 82.5 mu g/ml",

author = "R Tundis and Loizzo, {M R} and Statti, {G A} and B Deguin and R Amissah and Houghton, {P J} and F Menichini",

year = "2005",

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doi = "10.1080/13880200500301886",

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journal = "PHARMACEUTICAL BIOLOGY",

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T1 - Chemical composition of and inhibition of angiotensin-converting enzyme by Senecio samnitum huet

AU - Tundis, R

AU - Loizzo, M R

AU - Statti, G A

AU - Deguin, B

AU - Amissah, R

AU - Houghton, P J

AU - Menichini, F

PY - 2005/10

Y1 - 2005/10

N2 - Extracts of Senecio samnitum Huet and derived methyl ester of chlorogenic acid have been shown to inhibit angiotensin-converting enzyme (ACE) by using an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labeled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by high-performance liquid chromatography (HPLC). GC/MS and NMR identified compounds present within the studied S. samnitum extracts. The most effective fraction was obtained in ethyl acetate, which gave 52.56 +/- 0.23% (SD) inhibition at 300 mu g/ml. The major constituent of this fraction, the methyl ester of chlorogenic acid, showed significant ACE inhibition of 56.78 +/- 0.25% at a concentration of 82.5 mu g/ml

AB - Extracts of Senecio samnitum Huet and derived methyl ester of chlorogenic acid have been shown to inhibit angiotensin-converting enzyme (ACE) by using an in vitro bioassay based on the enzymatic cleavage of the chromophore-fluorophore labeled substrate dansyltriglycine into dansylglycine, which is quantitatively measured by high-performance liquid chromatography (HPLC). GC/MS and NMR identified compounds present within the studied S. samnitum extracts. The most effective fraction was obtained in ethyl acetate, which gave 52.56 +/- 0.23% (SD) inhibition at 300 mu g/ml. The major constituent of this fraction, the methyl ester of chlorogenic acid, showed significant ACE inhibition of 56.78 +/- 0.25% at a concentration of 82.5 mu g/ml

U2 - 10.1080/13880200500301886

DO - 10.1080/13880200500301886

M3 - Article

VL - 43

SP - 605

EP - 608

JO - PHARMACEUTICAL BIOLOGY

JF - PHARMACEUTICAL BIOLOGY

IS - 7

ER -

Chemical composition of and inhibition of angiotensin-converting enzyme by Senecio samnitum huet

Abstract

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