Chronic probiotic supplementation with or without glutamine does not influence the eHsp72 response to a multi-day ultra-endurance exercise event

TY - JOUR

T1 - Chronic probiotic supplementation with or without glutamine does not influence the eHsp72 response to a multi-day ultra-endurance exercise event

AU - Marshall, Hannah

AU - Chrismas, Bryna Catherine Rose

AU - Suckling, Craig Anthony

AU - Roberts, Justin D

AU - Foster, Josh

AU - Taylor, Lee

PY - 2017/8

Y1 - 2017/8

N2 - Probiotic and glutamine supplementation increases tissue Hsp72, but their influence on extracellular Hsp72 (eHsp72) has not been investigated. The aim of this study was to investigate the effect of chronic probiotic supplementation, with or without glutamine, on eHsp72 concentration before and after an ultramarathon. Thirty-two participants were split into 3 independent groups, where they ingested probiotic capsules (PRO; n = 11), probiotic + glutamine powder (PGLn; n = 10), or no supplementation (CON; n = 11), over a 12-week period prior to commencement of the Marathon des Sables (MDS). eHsp72 concentration in the plasma was measured at baseline, 7 days pre-race, 6-8 h post-race, and 7 days post-race. The MDS increased eHsp72 concentrations by 124% (F[1,3] = 22.716, p < 0.001), but there was no difference in the response between groups. Additionally, PRO or PGLn supplementation did not modify pre- or post-MDS eHsp72 concentrations compared with CON (p > 0.05). In conclusion, the MDS caused a substantial increase in eHsp72 concentration, indicating high levels of systemic stress. However, chronic PRO or PGLn supplementation did not affect eHsp72 compared with control pre- or post-MDS. Given the role of eHsp72 in immune activation, the commercially available supplements used in this study are unlikely to influence this cascade.

AB - Probiotic and glutamine supplementation increases tissue Hsp72, but their influence on extracellular Hsp72 (eHsp72) has not been investigated. The aim of this study was to investigate the effect of chronic probiotic supplementation, with or without glutamine, on eHsp72 concentration before and after an ultramarathon. Thirty-two participants were split into 3 independent groups, where they ingested probiotic capsules (PRO; n = 11), probiotic + glutamine powder (PGLn; n = 10), or no supplementation (CON; n = 11), over a 12-week period prior to commencement of the Marathon des Sables (MDS). eHsp72 concentration in the plasma was measured at baseline, 7 days pre-race, 6-8 h post-race, and 7 days post-race. The MDS increased eHsp72 concentrations by 124% (F[1,3] = 22.716, p < 0.001), but there was no difference in the response between groups. Additionally, PRO or PGLn supplementation did not modify pre- or post-MDS eHsp72 concentrations compared with CON (p > 0.05). In conclusion, the MDS caused a substantial increase in eHsp72 concentration, indicating high levels of systemic stress. However, chronic PRO or PGLn supplementation did not affect eHsp72 compared with control pre- or post-MDS. Given the role of eHsp72 in immune activation, the commercially available supplements used in this study are unlikely to influence this cascade.

KW - Adolescent

KW - Adult

KW - Anthropometry

KW - Diet

KW - Female

KW - Glutamine/administration & dosage

KW - HSP72 Heat-Shock Proteins/genetics

KW - Humans

KW - Male

KW - Middle Aged

KW - Oxygen Consumption

KW - Physical Endurance/drug effects

KW - Probiotics/administration & dosage

KW - Running

KW - Surveys and Questionnaires

KW - Young Adult

U2 - 10.1139/apnm-2017-0131

DO - 10.1139/apnm-2017-0131

M3 - Article

C2 - 28460195

SN - 1715-5312

VL - 42

SP - 876

EP - 883

JO - Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme

JF - Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme

IS - 8

ER -