Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei

F R Cagampang; F A Antoni; S M Smith; H D Piggins; C W Coen

Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei

F R Cagampang, F A Antoni, S M Smith, H D Piggins, C W Coen

King's College London

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.

Original language	English
Pages (from-to)	279-82
Number of pages	4
Journal	Brain Research Bulletin
Volume	810
Issue number	1-2
Publication status	Published - 9 Nov 1998

Keywords

Adenylate Cyclase
Animals
Autoradiography
Circadian Rhythm
DNA, Complementary
Histocytochemistry
In Situ Hybridization
Male
RNA, Messenger
Rats
Rats, Wistar
Suprachiasmatic Nucleus

Cite this

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title = "Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei",

abstract = "Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.",

keywords = "Adenylate Cyclase, Animals, Autoradiography, Circadian Rhythm, DNA, Complementary, Histocytochemistry, In Situ Hybridization, Male, RNA, Messenger, Rats, Rats, Wistar, Suprachiasmatic Nucleus",

author = "Cagampang, {F R} and Antoni, {F A} and Smith, {S M} and Piggins, {H D} and Coen, {C W}",

year = "1998",

month = nov,

day = "9",

language = "English",

volume = "810",

pages = "279--82",

journal = "Brain Research Bulletin",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1-2",

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TY - JOUR

T1 - Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei

AU - Cagampang, F R

AU - Antoni, F A

AU - Smith, S M

AU - Piggins, H D

AU - Coen, C W

PY - 1998/11/9

Y1 - 1998/11/9

N2 - Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.

AB - Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.

KW - Adenylate Cyclase

KW - Animals

KW - Autoradiography

KW - Circadian Rhythm

KW - DNA, Complementary

KW - Histocytochemistry

KW - In Situ Hybridization

KW - Male

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Suprachiasmatic Nucleus

M3 - Article

C2 - 9813369

SN - 0006-8993

VL - 810

SP - 279

EP - 282

JO - Brain Research Bulletin

JF - Brain Research Bulletin

IS - 1-2

ER -

Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei

Abstract

Keywords

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