Clathrin-Independent Trafficking of AMPA Receptors

Oleg Glebov, Cezar M. Tigaret, Jack R. Mellor, Jeremy M. Henley

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


Membrane trafficking of AMPA receptors (AMPARs) is critical for neuronal function and plasticity. Although rapid forms of AMPAR internalization during long-term depression (LTD) require clathrin and dynamin, the mechanisms governing constitutive AMPAR turnover and internalization of AMPARs during slow homeostatic forms of synaptic plasticity remain unexplored. Here, we show that, in contrast to LTD, constitutive AMPAR internalization and homeostatic AMPAR downscaling in rat neurons do not require dynamin or clathrin function. Instead, constitutive AMPAR trafficking is blocked by a Rac1 inhibitor and is regulated by a dynamic nonstructural pool of F-actin. Our findings reveal a novel role for neuronal clathrin-independent endocytosis controlled by actin dynamics and suggest that the interplay between different modes of receptor endocytosis provides for segregation between distinct modes of neuronal plasticity.
Original languageEnglish
Pages (from-to)4830-4836
Number of pages7
JournalJournal of neuroscience
Issue number12
Publication statusPublished - 25 Mar 2015


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